3jtr: Difference between revisions
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==Mutations in Cephalosporin Acylase Affecting Stability and Autoproteolysis== | |||
=== | <StructureSection load='3jtr' size='340' side='right' caption='[[3jtr]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3jtr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_sp. Pseudomonas sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JTR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JTR FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2adv|2adv]], [[2ae4|2ae4]], [[2ae3|2ae3]], [[3jtq|3jtq]]</td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutaryl-7-aminocephalosporanic-acid_acylase Glutaryl-7-aminocephalosporanic-acid acylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.93 3.5.1.93] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3jtr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jtr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3jtr RCSB], [http://www.ebi.ac.uk/pdbsum/3jtr PDBsum]</span></td></tr> | |||
</table> | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jt/3jtr_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Cephalosporin acylase (CA), a member of the N-terminal nucleophile hydrolase family, is activated through two steps of intramolecular autoproteolysis, the first mediated by a serine residue, and the second by a glutamate, which releases the pro-segment and produces an active enzyme. In this study, we have determined the crystal structures of mutants which could affect primary or secondary auto-cleavage and of sequential intermediates of a slow-processing mutant at 2.0-2.5A resolutions. The pro-segments of the mutants undergo dynamic conformational changes during activation and adopt surprisingly different loop conformations from one another. However, the autoproteolytic site was found to form a catalytically competent conformation with a solvent water molecule, which was essentially conserved in the CA mutants. | |||
Structural features of cephalosporin acylase reveal the basis of autocatalytic activation.,Cho KJ, Kim JK, Lee JH, Shin HJ, Park SS, Kim KH Biochem Biophys Res Commun. 2009 Dec 11;390(2):342-8. Epub 2009 Oct 2. PMID:19800869<ref>PMID:19800869</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Glutaryl-7-aminocephalosporanic-acid acylase]] | [[Category: Glutaryl-7-aminocephalosporanic-acid acylase]] | ||
[[Category: Pseudomonas sp | [[Category: Pseudomonas sp]] | ||
[[Category: Cho, K J | [[Category: Cho, K J]] | ||
[[Category: Kim, J K | [[Category: Kim, J K]] | ||
[[Category: Kim, K H | [[Category: Kim, K H]] | ||
[[Category: Lee, J H | [[Category: Lee, J H]] | ||
[[Category: Park, S S | [[Category: Park, S S]] | ||
[[Category: Shin, H J | [[Category: Shin, H J]] | ||
[[Category: Autoproteolysis]] | [[Category: Autoproteolysis]] | ||
[[Category: Cephalosporin acylase]] | [[Category: Cephalosporin acylase]] | ||
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
Revision as of 16:54, 18 December 2014
Mutations in Cephalosporin Acylase Affecting Stability and Autoproteolysis
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