3khs: Difference between revisions
From Proteopedia
Jump to navigationJump to search
m Protected "3khs" [edit=sysop:move=sysop] |
No edit summary |
||
| Line 1: | Line 1: | ||
==Crystal structure of grouper iridovirus purine nucleoside phosphorylase== | |||
=== | <StructureSection load='3khs' size='340' side='right' caption='[[3khs]], [[Resolution|resolution]] 2.38Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3khs]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Grouper_iridovirus Grouper iridovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KHS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KHS FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GIV43 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=127569 Grouper iridovirus])</td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Purine-nucleoside_phosphorylase Purine-nucleoside phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.1 2.4.2.1] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3khs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3khs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3khs RCSB], [http://www.ebi.ac.uk/pdbsum/3khs PDBsum]</span></td></tr> | |||
</table> | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kh/3khs_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Purine nucleoside phosphorylase (PNP) catalyzes the reversible phosphorolysis of purine ribonucleosides to the corresponding free bases and ribose 1-phosphate. The crystal structure of grouper iridovirus PNP (givPNP), corresponding to the first PNP gene to be found in a virus, was determined at 2.4 A resolution. The crystals belonged to space group R3, with unit-cell parameters a = 193.0, c = 105.6 A, and contained four protomers per asymmetric unit. The overall structure of givPNP shows high similarity to mammalian PNPs, having an alpha/beta structure with a nine-stranded mixed beta-barrel flanked by a total of nine alpha-helices. The predicted phosphate-binding and ribose-binding sites are occupied by a phosphate ion and a Tris molecule, respectively. The geometrical arrangement and hydrogen-bonding patterns of the phosphate-binding site are similar to those found in the human and bovine PNP structures. The enzymatic activity assay of givPNP on various substrates revealed that givPNP can only accept 6-oxopurine nucleosides as substrates, which is also suggested by its amino-acid composition and active-site architecture. All these results suggest that givPNP is a homologue of mammalian PNPs in terms of amino-acid sequence, molecular mass, substrate specificity and overall structure, as well as in the composition of the active site. | |||
Structure of grouper iridovirus purine nucleoside phosphorylase.,Kang YN, Zhang Y, Allan PW, Parker WB, Ting JW, Chang CY, Ealick SE Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):155-62. Epub 2010, Jan 22. PMID:20124695<ref>PMID:20124695</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
==See Also== | |||
*[[Purine nucleoside phosphorylase|Purine nucleoside phosphorylase]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Grouper iridovirus]] | [[Category: Grouper iridovirus]] | ||
[[Category: Purine-nucleoside phosphorylase]] | [[Category: Purine-nucleoside phosphorylase]] | ||
[[Category: Allan, P W | [[Category: Allan, P W]] | ||
[[Category: Chang, C Y | [[Category: Chang, C Y]] | ||
[[Category: Ealick, S E | [[Category: Ealick, S E]] | ||
[[Category: Kang, Y N | [[Category: Kang, Y N]] | ||
[[Category: Parker, W B | [[Category: Parker, W B]] | ||
[[Category: Ting, J W | [[Category: Ting, J W]] | ||
[[Category: Zhang, Y | [[Category: Zhang, Y]] | ||
[[Category: Alpha-beta structure]] | [[Category: Alpha-beta structure]] | ||
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
[[Category: Mixed beta-barrel]] | [[Category: Mixed beta-barrel]] | ||
