2v2k: Difference between revisions

Mycobacterium smegmatis ferredoxin FdxA, which has an orthologue, ferredoxin in Mycobacterium tuberculosis, FdxC, contains both one [3Fe-4S], and one [4Fe-4S] cluster. M. smegmatis FdxA has been shown to be a, preferred ferredoxin substrate of FprA [F. Fischer, D. Raimondi, A., Aliverti, G. Zanetti, Mycobacterium tuberculosis FprA, a novel bacterial, NADPH-ferredoxin reductase, Eur. J. Biochem. 269 (2002) 3005-3013], an, adrenodoxin reductase-like flavoprotein of M. tuberculosis, suggesting, that M. tuberculosis FdxC could be the physiological partner of the enzyme, in providing reducing power to the cytochromes P450. We report here the, crystal structure of FdxA at 1.6A resolution (R(factor) 16.5%, R(free), 20.2%). Besides providing an insight on protein architecture for this, 106-residue ferredoxin, our crystallographic investigation highlights, lability of the [4Fe-4S] center, which is shown to loose a Fe atom during, crystal growth. Due to their high similarity (87% sequence identity), the, structure here reported can be considered a valuable model for M., tuberculosis FdxC, thus representing a step forward in the study of the, complex mycobacterial redox pathways.

2V2K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis Mycobacterium smegmatis] with ACT and F3S as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2V2K OCA].  

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