1qwj: Difference between revisions
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[[Image:1qwj.gif|left|200px]] | [[Image:1qwj.gif|left|200px]] | ||
'''The Crystal Structure of Murine CMP-5-N-Acetylneuraminic Acid Synthetase''' | {{Structure | ||
|PDB= 1qwj |SIZE=350|CAPTION= <scene name='initialview01'>1qwj</scene>, resolution 2.8Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=NCC:CYTIDINE-5'-MONOPHOSPHATE-5-N-ACETYLNEURAMINIC ACID'>NCC</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/N-acylneuraminate_cytidylyltransferase N-acylneuraminate cytidylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.43 2.7.7.43] | |||
|GENE= Cmas ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]) | |||
}} | |||
'''The Crystal Structure of Murine CMP-5-N-Acetylneuraminic Acid Synthetase''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1QWJ is a [ | 1QWJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QWJ OCA]. | ||
==Reference== | ==Reference== | ||
The crystal structure of murine CMP-5-N-acetylneuraminic acid synthetase., Krapp S, Munster-Kuhnel AK, Kaiser JT, Huber R, Tiralongo J, Gerardy-Schahn R, Jacob U, J Mol Biol. 2003 Dec 5;334(4):625-37. PMID:[http:// | The crystal structure of murine CMP-5-N-acetylneuraminic acid synthetase., Krapp S, Munster-Kuhnel AK, Kaiser JT, Huber R, Tiralongo J, Gerardy-Schahn R, Jacob U, J Mol Biol. 2003 Dec 5;334(4):625-37. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14636592 14636592] | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: N-acylneuraminate cytidylyltransferase]] | [[Category: N-acylneuraminate cytidylyltransferase]] | ||
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[[Category: sugar-activating enzyme]] | [[Category: sugar-activating enzyme]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:43:37 2008'' | ||
Revision as of 11:43, 20 March 2008
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| 1qwj, resolution 2.8Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ligands: | NCC | ||||||||||||
| Gene: | Cmas (Mus musculus) | ||||||||||||
| Activity: | N-acylneuraminate cytidylyltransferase, with EC number 2.7.7.43 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
The Crystal Structure of Murine CMP-5-N-Acetylneuraminic Acid Synthetase
Overview
Sialic acids are activated by CMP-5-N-acetylneuraminic acid synthetase prior to their transfer onto oligo- or polysaccharides. Here, we present the crystal structure of the N-terminal catalytically active domain of the murine 5-N-acetylneuraminic acid synthetase in complex with the reaction product. In contrast to the previously solved structure of 5-N-acetylneuraminic acid synthetase from Neisseria meningitidis and the related CMP-KDO-synthetase of Escherichia coli, the murine enzyme is a tetramer, which was observed with the active sites closed. In this conformation a loop is shifted by 6A towards the active site and thus an essential arginine residue can participate in catalysis. Furthermore, a network of intermolecular salt-bridges and hydrogen bonds in the dimer as well as hydrophobic interfaces between two dimers indicate a cooperative behaviour of the enzyme. In addition, a complex regulation of the enzyme activity is proposed that includes phosphorylation and dephosphorylation.
About this Structure
1QWJ is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
The crystal structure of murine CMP-5-N-acetylneuraminic acid synthetase., Krapp S, Munster-Kuhnel AK, Kaiser JT, Huber R, Tiralongo J, Gerardy-Schahn R, Jacob U, J Mol Biol. 2003 Dec 5;334(4):625-37. PMID:14636592
Page seeded by OCA on Thu Mar 20 13:43:37 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Mus musculus
- N-acylneuraminate cytidylyltransferase
- Single protein
- Gerardy-Schahn, R.
- Huber, R.
- Jacob, U.
- Kaiser, J T.
- Krapp, S.
- Muenster-Kuehnel, A K.
- Tiralongo, J.
- NCC
- Cmp-5-n-acetylneuraminic acid synthetase
- Cmp-neu5ac
- Glycosylation
- Lipopolysaccharide biosynthesis
- Sialic acid
- Sugar-activating enzyme
