1r1b: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1r1b.jpg|left|200px]] | [[Image:1r1b.jpg|left|200px]] | ||
'''EPRS SECOND REPEATED ELEMENT, NMR, MINIMIZED AVERAGE STRUCTURE''' | {{Structure | ||
|PDB= 1r1b |SIZE=350|CAPTION= <scene name='initialview01'>1r1b</scene> | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''EPRS SECOND REPEATED ELEMENT, NMR, MINIMIZED AVERAGE STRUCTURE''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
1R1B is a [ | 1R1B is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R1B OCA]. | ||
==Reference== | ==Reference== | ||
A recurrent RNA-binding domain is appended to eukaryotic aminoacyl-tRNA synthetases., Cahuzac B, Berthonneau E, Birlirakis N, Guittet E, Mirande M, EMBO J. 2000 Feb 1;19(3):445-52. PMID:[http:// | A recurrent RNA-binding domain is appended to eukaryotic aminoacyl-tRNA synthetases., Cahuzac B, Berthonneau E, Birlirakis N, Guittet E, Mirande M, EMBO J. 2000 Feb 1;19(3):445-52. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10654942 10654942] | ||
[[Category: Cricetulus griseus]] | [[Category: Cricetulus griseus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 21: | Line 30: | ||
[[Category: trna synthetase (ligase)]] | [[Category: trna synthetase (ligase)]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:45:30 2008'' | ||
Revision as of 11:45, 20 March 2008
| |||||||||||||
| 1r1b | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
EPRS SECOND REPEATED ELEMENT, NMR, MINIMIZED AVERAGE STRUCTURE
Overview
Aminoacyl-tRNA synthetases of higher eukaryotes possess polypeptide extensions in contrast to their prokaryotic counterparts. These extra domains of poorly understood function are believed to be involved in protein-protein or protein-RNA interactions. Here we showed by gel retardation and filter binding experiments that the repeated units that build the linker region of the bifunctional glutamyl-prolyl-tRNA synthetase had a general RNA-binding capacity. The solution structure of one of these repeated motifs was also solved by NMR spectroscopy. One repeat is built around an antiparallel coiled-coil. Strikingly, the conserved lysine and arginine residues form a basic patch on one side of the structure, presenting a suitable docking surface for nucleic acids. Therefore, this repeated motif may represent a novel type of general RNA-binding domain appended to eukaryotic aminoacyl-tRNA synthetases to serve as a cis-acting tRNA-binding cofactor.
About this Structure
1R1B is a Single protein structure of sequence from Cricetulus griseus. Full crystallographic information is available from OCA.
Reference
A recurrent RNA-binding domain is appended to eukaryotic aminoacyl-tRNA synthetases., Cahuzac B, Berthonneau E, Birlirakis N, Guittet E, Mirande M, EMBO J. 2000 Feb 1;19(3):445-52. PMID:10654942
Page seeded by OCA on Thu Mar 20 13:45:30 2008
