1r5d: Difference between revisions
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'''X-ray structure of bovine seminal ribonuclease swapping dimer from a new crystal form''' | {{Structure | ||
|PDB= 1r5d |SIZE=350|CAPTION= <scene name='initialview01'>1r5d</scene>, resolution 2.5Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] | |||
|GENE= | |||
}} | |||
'''X-ray structure of bovine seminal ribonuclease swapping dimer from a new crystal form''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1R5D is a [ | 1R5D is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R5D OCA]. | ||
==Reference== | ==Reference== | ||
Population shift vs induced fit: the case of bovine seminal ribonuclease swapping dimer., Merlino A, Vitagliano L, Sica F, Zagari A, Mazzarella L, Biopolymers. 2004 Apr 15;73(6):689-95. PMID:[http:// | Population shift vs induced fit: the case of bovine seminal ribonuclease swapping dimer., Merlino A, Vitagliano L, Sica F, Zagari A, Mazzarella L, Biopolymers. 2004 Apr 15;73(6):689-95. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15048772 15048772] | ||
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Pancreatic ribonuclease]] | [[Category: Pancreatic ribonuclease]] | ||
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[[Category: ligand binding]] | [[Category: ligand binding]] | ||
[[Category: population shift]] | [[Category: population shift]] | ||
[[Category: protein | [[Category: protein dynamic]] | ||
[[Category: protein structure-function]] | [[Category: protein structure-function]] | ||
[[Category: | [[Category: ribonuclease]] | ||
[[Category: x-ray diffraction]] | [[Category: x-ray diffraction]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:47:07 2008'' | ||
Revision as of 11:47, 20 March 2008
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| 1r5d, resolution 2.5Å | |||||||||||||
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| Activity: | Pancreatic ribonuclease, with EC number 3.1.27.5 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
X-ray structure of bovine seminal ribonuclease swapping dimer from a new crystal form
Overview
Bovine seminal ribonuclease (BS-RNase) is a unique member of the pancreatic-like ribonuclease superfamily. This enzyme exists as two conformational isomers with distinctive biological properties. The structure of the major isomer is characterized by the swapping of the N-terminal segment (MxM BS-RNase). In this article, the crystal structures of the ligand-free MxM BS-RNase and its complex with 2'-deoxycitidylyl(3',5')-2'-deoxyadenosine derived from isomorphous crystals have been refined. Interestingly, the comparison between this novel ligand-free form and the previously published sulfate-bound structure reveals significant differences. In particular, the ligand-free MxM BS-RNase is closer to the structure of MxM BS-RNase productive complexes than to the sulfate-bound form. These results reveal that MxM BS-RNase presents a remarkable flexibility, despite the structural constraints of the interchain disulfide bridges and the swapping of the N-terminal helices. These findings have important implications to the ligand binding mechanism of MxM BS-RNase. Indeed, a population shift rather than a substrate-induced conformational transition may occur in the MxM BS-RNase ligand binding process.
About this Structure
1R5D is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
Population shift vs induced fit: the case of bovine seminal ribonuclease swapping dimer., Merlino A, Vitagliano L, Sica F, Zagari A, Mazzarella L, Biopolymers. 2004 Apr 15;73(6):689-95. PMID:15048772
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