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{{STRUCTURE_3vws|  PDB=3vws  |  SCENE=  }}
==Dengue serotype 3 RNA-dependent RNA polymerase bound to NITD-107==
===Dengue serotype 3 RNA-dependent RNA polymerase bound to NITD-107===
<StructureSection load='3vws' size='340' side='right' caption='[[3vws]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
{{ABSTRACT_PUBMED_23408636}}
== Structural highlights ==
<table><tr><td colspan='2'>[[3vws]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_3 Dengue virus 3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VWS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VWS FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=VWS:5-{[(4-CHLOROPHENYL)SULFONYL]AMINO}-2-METHYL-1-BENZOFURAN-3-CARBOXYLIC+ACID'>VWS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vws OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vws RCSB], [http://www.ebi.ac.uk/pdbsum/3vws PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We report a highly reproducible method to crystallize the RNA-dependent RNA polymerase (RdRp) domain of dengue virus serotype-3 (DENV-3), allowing structure refinement to 1.79 A resolution and revealing amino acids not seen previously. We also present a DENV-3 polymerase/inhibitor co-crystal structure at 2.1 A resolution. The inhibitor binds to the RdRp as a dimer and causes conformational changes in the protein. The improved crystallization conditions and new structural information should accelerate structure-based drug discovery.


==Function==
Conformational flexibility of the dengue virus RNA-dependent RNA polymerase revealed by a complex with an inhibitor.,Noble CG, Lim SP, Chen YL, Liew CW, Yap L, Lescar J, Shi PY J Virol. 2013 Feb 13. PMID:23408636<ref>PMID:23408636</ref>
[[http://www.uniprot.org/uniprot/Q6DLV0_9FLAV Q6DLV0_9FLAV]] Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).[SAAS:SAAS000336_004_099774]


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[3vws]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_3 Dengue virus 3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VWS OCA].
</div>
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:023408636</ref><references group="xtra"/><references/>
__TOC__
</StructureSection>
[[Category: Dengue virus 3]]
[[Category: Dengue virus 3]]
[[Category: RNA-directed RNA polymerase]]
[[Category: RNA-directed RNA polymerase]]
[[Category: Lescar, J.]]
[[Category: Lescar, J]]
[[Category: Noble, C G.]]
[[Category: Noble, C G]]
[[Category: Rna polymerase]]
[[Category: Rna polymerase]]
[[Category: Rna-dependent rna polymerase]]
[[Category: Rna-dependent rna polymerase]]
[[Category: Transferase-transferase inhibitor complex]]
[[Category: Transferase-transferase inhibitor complex]]
[[Category: Viral protein]]
[[Category: Viral protein]]
Retrieved from "https://proteopedia.org/3vws"