3vv1: Difference between revisions
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==Crystal Structure of Caenorhabditis elegans galectin LEC-6== | |||
=== | <StructureSection load='3vv1' size='340' side='right' caption='[[3vv1]], [[Resolution|resolution]] 1.50Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3vv1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VV1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VV1 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lec-6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 Caenorhabditis elegans])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vv1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vv1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vv1 RCSB], [http://www.ebi.ac.uk/pdbsum/3vv1 PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Galectins are a group of lectins that can bind carbohydrate chains containing beta-galactoside units. LEC-6, a member of galectins of Caenorhabditis elegans, binds fucose-containing saccharides. We solved the crystal structure of LEC-6 in complex with galactose-beta1,4-fucose (Galbeta1-4Fuc) at 1.5 A resolution. The overall structure of the protein and the identities of the amino-acid residues binding to the disaccharide are similar to those of other galectins. However, further structural analysis and multiple sequence alignment between LEC-6 and other galectins indicate that a glutamic acid residue (Glu67) is important for the preferential binding between LEC-6 and the fucose moiety of the Galbeta1-4Fuc unit. Frontal affinity chromatography analysis indicated that the affinities of E67D and E67A mutants for Galbeta1-4Fuc are lower than that of wild-type LEC-6. Furthermore, the affinities of Glu67 mutants for an endogenous oligosaccharide, which contains a Galbeta1-4Fuc unit, are drastically reduced relative to that of the wild-type protein. We conclude that the Glu67 in the oligosaccharide-binding site assists the recognition of the fucose moiety by LEC-6. | |||
Structural basis of preferential binding of fucose-containing saccharide by the Caenorhabditis elegans galectin LEC-6.,Makyio H, Takeuchi T, Tamura M, Nishiyama K, Takahashi H, Natsugari H, Arata Y, Kasai KI, Yamada Y, Wakatsuki S, Kato R Glycobiology. 2013 Mar 28. PMID:23481096<ref>PMID:23481096</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Caenorhabditis elegans]] | [[Category: Caenorhabditis elegans]] | ||
[[Category: Arata, Y | [[Category: Arata, Y]] | ||
[[Category: Kasai, K | [[Category: Kasai, K]] | ||
[[Category: Kato, R | [[Category: Kato, R]] | ||
[[Category: Makyio, H | [[Category: Makyio, H]] | ||
[[Category: Natsugari, H | [[Category: Natsugari, H]] | ||
[[Category: Nishiyama, K | [[Category: Nishiyama, K]] | ||
[[Category: Takahashi, H | [[Category: Takahashi, H]] | ||
[[Category: Takeuchi, T | [[Category: Takeuchi, T]] | ||
[[Category: Tamura, M | [[Category: Tamura, M]] | ||
[[Category: Wakatsuki, S | [[Category: Wakatsuki, S]] | ||
[[Category: Yamada, Y | [[Category: Yamada, Y]] | ||
[[Category: 4-fucose binding]] | [[Category: 4-fucose binding]] | ||
[[Category: Galactose-1]] | [[Category: Galactose-1]] | ||
[[Category: Galectin]] | [[Category: Galectin]] | ||
[[Category: Sugar binding protein]] | [[Category: Sugar binding protein]] | ||
Revision as of 06:47, 21 December 2014
Crystal Structure of Caenorhabditis elegans galectin LEC-6
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