422d: Difference between revisions

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~~{{STRUCTURE_422d| PDB=422d | SCENE= }}~~

==5'-R(*GP*AP*UP*CP*AP*CP*UP*UP*CP*GP*GP*U)-3'==

===5'-R(*GP*AP*UP*CP*AP*CP*UP*UP*CP*GP*GP*U)-3'===

~~{{ABSTRACT_PUBMED_14684886}}~~

== Structural highlights ==

<table><tr><td colspan='2'>[[422d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=422D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=422D FirstGlance]. <br>

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=422d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=422d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=422d RCSB], [http://www.ebi.ac.uk/pdbsum/422d PDBsum]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

The crystal structure of an RNA dodecamer, r(GAUCACUUCGGU), was solved at 2.6 A resolution by the molecular-replacement method and refined to an R(work) of 18.8% (R(free) = 22.8%) using 2494 reflections. The dodecamer crystallized in the monoclinic space group C2, with unit-cell parameters a = 71.34, b = 39.98, c = 32.47 A, beta = 104.7 degrees and two independent strands in the asymmetric unit. The dodecamer adopts an octamer duplex structure with four 5'-overhang residues (G1A2U3C4), which form Watson-Crick base pairs with another four 5'-overhang residues of a symmetry-related duplex. The octamer duplex (ACUUCGGU) contains at its center four mismatched base pairs flanked by two Watson-Crick base pairs. The mismatched bases form two G.U wobble base pairs at the ends and two U.C base pairs at the center, with one base-base hydrogen bond N4(C).O4(U) and a water bridge connecting the N(3) of the cytosine and uridine. The present study reinforces the concept of the stability of the conformation of UUCG in RNA double-helical structures.

~~==About this~~ Structure==

Structure of the dodecamer r(GAUCACUUCGGU) with four 5'-overhang nucleotides.,Eswaramoorthy S, Rao ST, Pan B, Sundaralingam M Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):8-12. Epub 2003 Dec, 18. PMID:14684886<ref>PMID:14684886</ref>

~~[[422d]] is a 2 chain structure~~. ~~Full crystallographic information is available from [http~~:~~//oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=422D OCA].~~

==~~Reference~~==

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

~~<ref group="xtra">PMID:014684886</ref>~~<references ~~group="xtra"~~/><~~references~~/>

</div>

[[Category: Eswaramoorthy, S.]]

== References ==

[[Category: Rao, S T.]]

[[Category: Sundaralingam, M.]]

__TOC__

</StructureSection>

[[Category: Eswaramoorthy, S]]

[[Category: Rao, S T]]

[[Category: Sundaralingam, M]]

[[Category: Double helix]]

[[Category: Rna]]

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@@ Line 1: / Line 1: @@
-{{STRUCTURE_422d|  PDB=422d  |  SCENE=  }}
+==5'-R(*GP*AP*UP*CP*AP*CP*UP*UP*CP*GP*GP*U)-3'==
-===5'-R(*GP*AP*UP*CP*AP*CP*UP*UP*CP*GP*GP*U)-3'===
+<StructureSection load='422d' size='340' side='right' caption='[[422d]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
-{{ABSTRACT_PUBMED_14684886}}
+== Structural highlights ==
+<table><tr><td colspan='2'>[[422d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=422D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=422D FirstGlance]. <br>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=422d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=422d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=422d RCSB], [http://www.ebi.ac.uk/pdbsum/422d PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The crystal structure of an RNA dodecamer, r(GAUCACUUCGGU), was solved at 2.6 A resolution by the molecular-replacement method and refined to an R(work) of 18.8% (R(free) = 22.8%) using 2494 reflections. The dodecamer crystallized in the monoclinic space group C2, with unit-cell parameters a = 71.34, b = 39.98, c = 32.47 A, beta = 104.7 degrees and two independent strands in the asymmetric unit. The dodecamer adopts an octamer duplex structure with four 5'-overhang residues (G1A2U3C4), which form Watson-Crick base pairs with another four 5'-overhang residues of a symmetry-related duplex. The octamer duplex (ACUUCGGU) contains at its center four mismatched base pairs flanked by two Watson-Crick base pairs. The mismatched bases form two G.U wobble base pairs at the ends and two U.C base pairs at the center, with one base-base hydrogen bond N4(C).O4(U) and a water bridge connecting the N(3) of the cytosine and uridine. The present study reinforces the concept of the stability of the conformation of UUCG in RNA double-helical structures.
-==About this Structure==
+Structure of the dodecamer r(GAUCACUUCGGU) with four 5'-overhang nucleotides.,Eswaramoorthy S, Rao ST, Pan B, Sundaralingam M Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):8-12. Epub 2003 Dec, 18. PMID:14684886<ref>PMID:14684886</ref>
-[[422d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=422D OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<ref group="xtra">PMID:014684886</ref><references group="xtra"/><references/>
+</div>
-[[Category: Eswaramoorthy, S.]]
+== References ==
-[[Category: Rao, S T.]]
+<references/>
-[[Category: Sundaralingam, M.]]
+__TOC__
+</StructureSection>
+[[Category: Eswaramoorthy, S]]
+[[Category: Rao, S T]]
+[[Category: Sundaralingam, M]]
 [[Category: Double helix]]
 [[Category: Rna]]