4bx9: Difference between revisions

Line 1:

~~{{STRUCTURE_4bx9| PDB=4bx9 | SCENE= }}~~

==Human Vps33A in complex with a fragment of human Vps16==

===Human Vps33A in complex with a fragment of human Vps16===

~~{{ABSTRACT_PUBMED_23901104}}~~

== Structural highlights ==

<table><tr><td colspan='2'>[[4bx9]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BX9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BX9 FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=LMR:(2S)-2-HYDROXYBUTANEDIOIC+ACID'>LMR</scene>, <scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene></td></tr>

<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4bx8|4bx8]]</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bx9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bx9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bx9 RCSB], [http://www.ebi.ac.uk/pdbsum/4bx9 PDBsum]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

The multisubunit homotypic fusion and vacuole protein sorting (HOPS) membrane-tethering complex is required for late endosome-lysosome and autophagosome-lysosome fusion in mammals. We have determined the crystal structure of the human HOPS subunit Vps33A, confirming its identity as a Sec1/Munc18 family member. We show that HOPS subunit Vps16 recruits Vps33A to the human HOPS complex and that residues 642-736 are necessary and sufficient for this interaction, and we present the crystal structure of Vps33A in complex with Vps16(642-736). Mutations at the binding interface disrupt the Vps33A-Vps16 interaction both in vitro and in cells, preventing recruitment of Vps33A to the HOPS complex. The Vps33A-Vps16 complex provides a structural framework for studying the association between Sec1/Munc18 proteins and tethering complexes.

~~==Function==~~

Structural basis of Vps33A recruitment to the human HOPS complex by Vps16.,Graham SC, Wartosch L, Gray SR, Scourfield EJ, Deane JE, Luzio JP, Owen DJ Proc Natl Acad Sci U S A. 2013 Jul 30. PMID:23901104<ref>PMID:23901104</ref>

~~[[http://www~~.~~uniprot~~.org/uniprot/VP33A_HUMAN VP33A_HUMAN]] May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes (By similarity). ~~[[http~~://www.uniprot.org/uniprot/VPS16_HUMAN VPS16_HUMAN]] May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.

~~==About this Structure==~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

~~[[4bx9]] is~~ a ~~3 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=4BX9 OCA].~~

</div>

== References ==

==~~Reference~~==

~~<ref group="xtra">PMID:023901104</ref>~~<references ~~group="xtra"~~/><~~references~~/>

__TOC__

</StructureSection>

[[Category: Homo sapiens]]

[[Category: Deane, J E.]]

[[Category: Deane, J E]]

[[Category: Graham, S C.]]

[[Category: Graham, S C]]

[[Category: Gray, S R.]]

[[Category: Gray, S R]]

[[Category: Luzio, J P.]]

[[Category: Luzio, J P]]

[[Category: Owen, D J.]]

[[Category: Owen, D J]]

[[Category: Scourfield, E J.]]

[[Category: Scourfield, E J]]

[[Category: Wartosch, L.]]

[[Category: Wartosch, L]]

[[Category: Hop]]

[[Category: Membrane trafficking]]

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@@ Line 1: / Line 1: @@
-{{STRUCTURE_4bx9|  PDB=4bx9  |  SCENE=  }}
+==Human Vps33A in complex with a fragment of human Vps16==
-===Human Vps33A in complex with a fragment of human Vps16===
+<StructureSection load='4bx9' size='340' side='right' caption='[[4bx9]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
-{{ABSTRACT_PUBMED_23901104}}
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4bx9]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BX9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BX9 FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=LMR:(2S)-2-HYDROXYBUTANEDIOIC+ACID'>LMR</scene>, <scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4bx8|4bx8]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bx9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bx9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bx9 RCSB], [http://www.ebi.ac.uk/pdbsum/4bx9 PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The multisubunit homotypic fusion and vacuole protein sorting (HOPS) membrane-tethering complex is required for late endosome-lysosome and autophagosome-lysosome fusion in mammals. We have determined the crystal structure of the human HOPS subunit Vps33A, confirming its identity as a Sec1/Munc18 family member. We show that HOPS subunit Vps16 recruits Vps33A to the human HOPS complex and that residues 642-736 are necessary and sufficient for this interaction, and we present the crystal structure of Vps33A in complex with Vps16(642-736). Mutations at the binding interface disrupt the Vps33A-Vps16 interaction both in vitro and in cells, preventing recruitment of Vps33A to the HOPS complex. The Vps33A-Vps16 complex provides a structural framework for studying the association between Sec1/Munc18 proteins and tethering complexes.
-==Function==
+Structural basis of Vps33A recruitment to the human HOPS complex by Vps16.,Graham SC, Wartosch L, Gray SR, Scourfield EJ, Deane JE, Luzio JP, Owen DJ Proc Natl Acad Sci U S A. 2013 Jul 30. PMID:23901104<ref>PMID:23901104</ref>
-[[http://www.uniprot.org/uniprot/VP33A_HUMAN VP33A_HUMAN]] May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes (By similarity). [[http://www.uniprot.org/uniprot/VPS16_HUMAN VPS16_HUMAN]] May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.
-==About this Structure==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[4bx9]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BX9 OCA].
+</div>
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:023901104</ref><references group="xtra"/><references/>
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Deane, J E.]]
+[[Category: Deane, J E]]
-[[Category: Graham, S C.]]
+[[Category: Graham, S C]]
-[[Category: Gray, S R.]]
+[[Category: Gray, S R]]
-[[Category: Luzio, J P.]]
+[[Category: Luzio, J P]]
-[[Category: Owen, D J.]]
+[[Category: Owen, D J]]
-[[Category: Scourfield, E J.]]
+[[Category: Scourfield, E J]]
-[[Category: Wartosch, L.]]
+[[Category: Wartosch, L]]
 [[Category: Hop]]
 [[Category: Membrane trafficking]]