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{{STRUCTURE_4dcn|  PDB=4dcn  |  SCENE=  }}
==Crystal Structure Analysis of the Arfaptin2 BAR domain in Complex with ARL1==
===Crystal Structure Analysis of the Arfaptin2 BAR domain in Complex with ARL1===
<StructureSection load='4dcn' size='340' side='right' caption='[[4dcn]], [[Resolution|resolution]] 3.01&Aring;' scene=''>
{{ABSTRACT_PUBMED_22679020}}
== Structural highlights ==
<table><tr><td colspan='2'>[[4dcn]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DCN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DCN FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1moz|1moz]], [[1upt|1upt]], [[1i49|1i49]], [[1i4l|1i4l]], [[3dyt|3dyt]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ARL1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), ARFIP2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dcn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dcn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dcn RCSB], [http://www.ebi.ac.uk/pdbsum/4dcn PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Membrane-sculpting BAR (Bin/Amphiphysin/Rvs) domains form a crescent-shaped homodimer that can sense and induce membrane curvature through its positively charged concave face. We have recently shown that Arfaptin-2, which was originally identified as a binding partner for the Arf and Rac1 GTPases, binds to Arl1 through its BAR domain and is recruited onto Golgi membranes. There, Arfaptin-2 induces membrane tubules. Here, we report the crystal structure of the Arfaptin-2 BAR homodimer in complex with two Arl1 molecules bound symmetrically to each side, leaving the concave face open for membrane association. The overall structure of the Arl1.Arfaptin-2 BAR complex closely resembles that of the PX-BAR domain of sorting nexin 9, suggesting similar mechanisms underlying BAR domain targeting to specific organellar membranes. The Arl1.Arfaptin-2 BAR structure suggests that one of the two Arl1 molecules competes with Rac1, which binds to the concave face of the Arfaptin-2 BAR homodimer and may hinder its membrane association.


==Function==
Structural basis for membrane binding specificity of the Bin/Amphiphysin/Rvs (BAR) domain of Arfaptin-2 determined by Arl1 GTPase.,Nakamura K, Man Z, Xie Y, Hanai A, Makyio H, Kawasaki M, Kato R, Shin HW, Nakayama K, Wakatsuki S J Biol Chem. 2012 Jul 20;287(30):25478-89. doi: 10.1074/jbc.M112.365783. Epub, 2012 Jun 7. PMID:22679020<ref>PMID:22679020</ref>
[[http://www.uniprot.org/uniprot/ARL1_HUMAN ARL1_HUMAN]] GTP-binding protein that has very low efficiency as allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Can activate phospholipase D with very low efficiency. Important for normal function of the Golgi apparatus.<ref>PMID:9624189</ref> [[http://www.uniprot.org/uniprot/ARFP2_HUMAN ARFP2_HUMAN]] Putative target protein of ADP-ribosylation factor. Involved in membrane ruffling.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[4dcn]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DCN OCA].
</div>
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:022679020</ref><references group="xtra"/><references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Kato, R.]]
[[Category: Kato, R]]
[[Category: Kawasaki, M.]]
[[Category: Kawasaki, M]]
[[Category: Nakamura, K.]]
[[Category: Nakamura, K]]
[[Category: Wakatsuki, S.]]
[[Category: Wakatsuki, S]]
[[Category: Xie, Y.]]
[[Category: Xie, Y]]
[[Category: Bar domain]]
[[Category: Bar domain]]
[[Category: Membrane deformation]]
[[Category: Membrane deformation]]
[[Category: Protein binding-signaling protein complex]]
[[Category: Protein binding-signaling protein complex]]
[[Category: Small gtpase effector complex]]
[[Category: Small gtpase effector complex]]
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