4blf: Difference between revisions
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==Variable internal flexibility characterizes the helical capsid formed by Agrobacterium VirE2 protein on single-stranded DNA.== | |||
<StructureSection load='4blf' size='340' side='right' caption='[[4blf]], [[Resolution|resolution]] 20.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4blf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BLF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BLF FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4blf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4blf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4blf RCSB], [http://www.ebi.ac.uk/pdbsum/4blf PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Agrobacterium is known for gene transfer to plants. In addition to a linear ssDNA oligonucleotide, Agrobacterium tumefaciens secretes an abundant ssDNA-binding effector, VirE2. In many ways VirE2 adapts the conjugation mechanism to transform the eukaryotic host. The crystal structure of VirE2 shows two compact domains joined by a flexible linker. Bound to ssDNA, VirE2 forms an ordered solenoidal shell, or capsid known as the T-complex. Here, we present a three-dimensional reconstruction of the VirE2-ssDNA complex using cryo-electron microscopy and iterative helical real-space reconstruction. High-resolution refinement was not possible due to inherent heterogeneity in the protein structure. By a combination of computational modeling, chemical modifications, mass spectroscopy, and electron paramagnetic resonance, we found that the N-terminal domain is tightly constrained by both tangential and longitudinal links, while the C terminus is weakly constrained. The quaternary structure is thus rigidly assembled while remaining locally flexible. This flexibility may be important in accommodating substrates without sequence specificity. | |||
Variable Internal Flexibility Characterizes the Helical Capsid Formed by Agrobacterium VirE2 Protein on Single-Stranded DNA.,Bharat TA, Zbaida D, Eisenstein M, Frankenstein Z, Mehlman T, Weiner L, Sorzano CO, Barak Y, Albeck S, Briggs JA, Wolf SG, Elbaum M Structure. 2013 Jun 11. pii: S0969-2126(13)00157-3. doi:, 10.1016/j.str.2013.04.027. PMID:23769668<ref>PMID:23769668</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
== | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Agrobacterium tumefaciens]] | [[Category: Agrobacterium tumefaciens]] | ||
[[Category: Albeck, S | [[Category: Albeck, S]] | ||
[[Category: Barak, Y | [[Category: Barak, Y]] | ||
[[Category: Bharat, T A.M | [[Category: Bharat, T A.M]] | ||
[[Category: Briggs, J A.G | [[Category: Briggs, J A.G]] | ||
[[Category: Eisenstein, M | [[Category: Eisenstein, M]] | ||
[[Category: Elbaum, M | [[Category: Elbaum, M]] | ||
[[Category: Frankenstein, Z | [[Category: Frankenstein, Z]] | ||
[[Category: Mehlman, T | [[Category: Mehlman, T]] | ||
[[Category: Sorzano, C O.S | [[Category: Sorzano, C O.S]] | ||
[[Category: Weiner, L | [[Category: Weiner, L]] | ||
[[Category: Wolf, S G | [[Category: Wolf, S G]] | ||
[[Category: Zbaida, D | [[Category: Zbaida, D]] | ||
[[Category: Agrobacterium]] | [[Category: Agrobacterium]] | ||
[[Category: Dna binding protein]] | [[Category: Dna binding protein]] | ||
[[Category: Helical reconstruction]] | [[Category: Helical reconstruction]] | ||
[[Category: Tcomplex]] | [[Category: Tcomplex]] | ||
Revision as of 09:48, 21 December 2014
Variable internal flexibility characterizes the helical capsid formed by Agrobacterium VirE2 protein on single-stranded DNA.
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