4beh: Difference between revisions
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==Solution structure of human ribosomal protein P1.P2 heterodimer== | |||
<StructureSection load='4beh' size='340' side='right' caption='[[4beh]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4beh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BEH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BEH FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4beh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4beh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4beh RCSB], [http://www.ebi.ac.uk/pdbsum/4beh PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Lateral ribosomal stalk is responsible for binding and recruiting translation factors during protein synthesis. The eukaryotic stalk consists of one P0 protein with two copies of P1*P2 heterodimers to form a P0(P1*P2)2 pentameric P-complex. Here, we have solved the structure of full-length P1*P2 by nuclear magnetic resonance spectroscopy. P1 and P2 dimerize via their helical N-terminal domains, whereas the C-terminal tails of P1*P2 are unstructured and can extend up to approximately 125 A away from the dimerization domains. 15N relaxation study reveals that the C-terminal tails are flexible, having a much faster internal mobility than the N-terminal domains. Replacement of prokaryotic L10(L7/L12)4/L11 by eukaryotic P0(P1*P2)2/eL12 rendered Escherichia coli ribosome, which is insensitive to trichosanthin (TCS), susceptible to depurination by TCS and the C-terminal tail was found to be responsible for this depurination. Truncation and insertion studies showed that depurination of hybrid ribosome is dependent on the length of the proline-alanine rich hinge region within the C-terminal tail. All together, we propose a model that recruitment of TCS to the sarcin-ricin loop required the flexible C-terminal tail, and the proline-alanine rich hinge region lengthens this C-terminal tail, allowing the tail to sweep around the ribosome to recruit TCS. | |||
Solution structure of human P1*P2 heterodimer provides insights into the role of eukaryotic stalk in recruiting the ribosome-inactivating protein trichosanthin to the ribosome.,Lee KM, Yusa K, Chu LO, Wing-Heng Yu C, Oono M, Miyoshi T, Ito K, Shaw PC, Wong KB, Uchiumi T Nucleic Acids Res. 2013 Jul 26. PMID:23892290<ref>PMID:23892290</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== | ==See Also== | ||
*[[Ribosomal protein P1|Ribosomal protein P1]] | |||
*[[Ribosomal protein P2|Ribosomal protein P2]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Chu, L O | [[Category: Chu, L O]] | ||
[[Category: Ito, K | [[Category: Ito, K]] | ||
[[Category: Lee, K M | [[Category: Lee, K M]] | ||
[[Category: Miyoshi, T | [[Category: Miyoshi, T]] | ||
[[Category: Oono, M | [[Category: Oono, M]] | ||
[[Category: Shaw, P C | [[Category: Shaw, P C]] | ||
[[Category: Uchiumi, T | [[Category: Uchiumi, T]] | ||
[[Category: Wong, K B | [[Category: Wong, K B]] | ||
[[Category: Yu, C Wing-Heng | [[Category: Yu, C Wing-Heng]] | ||
[[Category: Yusa, K | [[Category: Yusa, K]] | ||
[[Category: Ribosome]] | [[Category: Ribosome]] | ||
[[Category: Stalk]] | [[Category: Stalk]] | ||
[[Category: Translation]] | [[Category: Translation]] | ||
