4gf3: Difference between revisions
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==Structure of a SycH-YopH Chaperone-Effector Complex== | |||
<StructureSection load='4gf3' size='340' side='right' caption='[[4gf3]], [[Resolution|resolution]] 1.90Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4gf3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Yersinia_enterocolitica Yersinia enterocolitica] and [http://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GF3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GF3 FirstGlance]. <br> | |||
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sycH, YP_pCD87, YPCD1.95c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=632 Yersinia pestis]), yopH, yop51 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=630 Yersinia enterocolitica])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gf3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gf3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gf3 RCSB], [http://www.ebi.ac.uk/pdbsum/4gf3 PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Yersinia pestis injects numerous bacterial proteins into host cells through an organic nanomachine called the type 3 secretion system. One such substrate is the tyrosine phosphatase YopH, which requires an interaction with a cognate chaperone in order to be effectively injected. Here, the first crystal structure of a SycH-YopH complex is reported, determined to 1.9 A resolution. The structure reveals the presence of (i) a nonglobular polypeptide in YopH, (ii) a so-called beta-motif in YopH and (iii) a conserved hydrophobic patch in SycH that recognizes the beta-motif. Biochemical studies establish that the beta-motif is critical to the stability of this complex. Finally, since previous work has shown that the N-terminal portion of YopH adopts a globular fold that is functional in the host cell, aspects of how this polypeptide adopts radically different folds in the host and in the bacterial environments are analysed. | |||
Context-dependent protein folding of a virulence peptide in the bacterial and host environments: structure of an SycH-YopH chaperone-effector complex.,Vujanac M, Stebbins CE Acta Crystallogr D Biol Crystallogr. 2013 Apr;69(Pt 4):546-54. doi:, 10.1107/S0907444912051086. Epub 2013 Mar 9. PMID:23519663<ref>PMID:23519663</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
==See Also== | |||
*[[Tyrosine phosphatase|Tyrosine phosphatase]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Yersinia enterocolitica]] | [[Category: Yersinia enterocolitica]] | ||
[[Category: Yersinia pestis]] | [[Category: Yersinia pestis]] | ||
[[Category: Stebbins, C E | [[Category: Stebbins, C E]] | ||
[[Category: Vujanac, M | [[Category: Vujanac, M]] | ||
[[Category: Chaperone-chaperone effector complex]] | [[Category: Chaperone-chaperone effector complex]] | ||
[[Category: T3ss chaperone]] | [[Category: T3ss chaperone]] | ||
Revision as of 10:50, 21 December 2014
Structure of a SycH-YopH Chaperone-Effector Complex
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