4g33: Difference between revisions
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==Crystal Structure of a Phospholipid-Lipoxygenase Complex from Pseudomonas aeruginosa at 2.0 A (C2221)== | |||
<StructureSection load='4g33' size='340' side='right' caption='[[4g33]], [[Resolution|resolution]] 2.03Å' scene=''> | |||
{ | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4g33]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G33 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G33 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZPE:(2R)-3-{[(S)-(2-AMINOETHOXY)(HYDROXY)PHOSPHORYL]OXY}-2-(TETRADEC-5-ENOYLOXY)PROPYL+(11Z)-OCTADEC-11-ENOATE'>ZPE</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4g32|4g32]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lox ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g33 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g33 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4g33 RCSB], [http://www.ebi.ac.uk/pdbsum/4g33 PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Lipoxygenases (LOXs), which are essential in eukaryotes, have no confirmed function in prokaryotes that are devoid of polyunsaturated fatty acids. The structure of a secretable LOX from Pseudomonas aeruginosa (Pa_LOX), the first available from a prokaryote, presents significant differences with respect to eukaryotic LOXs, including a cluster of helices acting as a lid to the active center. The mobility of the lid and the structural variability of the N-terminal region of Pa_LOX was confirmed by comparing 2 crystal forms. The binding pocket contains a phosphatidylethanolamine phospholipid with branches of 18 (sn-1) and 14/16 (sn-2) carbon atoms in length. Carbon atoms from the sn-1 chain approach the catalytic iron in a manner that sheds light on how the enzymatic reaction might proceed. The findings in these studies suggest that Pa_LOX has the capacity to extract and modify unsaturated phospholipids from eukaryotic membranes, allowing this LOX to play a role in the interaction of P. aeruginosa with host cells.-Garreta, A., Val-Moraes, S. P., Garcia-Fernandez, Q., Montserrat Busquets, C. J., Oliver, A., Ortiz, A., Gaffney, B. J., Fita, I., Manresa, A., Carpena, X. Structure and interaction with phospholipids of a prokaryotic lipoxygenase from Pseudomonas aeruginosa. | |||
Structure and interaction with phospholipids of a prokaryotic lipoxygenase from Pseudomonas aeruginosa.,Garreta A, Val-Moraes SP, Garcia-Fernandez Q, Busquets M, Juan C, Oliver A, Ortiz A, Gaffney BJ, Fita I, Manresa A, Carpena X FASEB J. 2013 Aug 30. PMID:23985801<ref>PMID:23985801</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
== | <references/> | ||
__TOC__ | |||
[[Category: Carpena, X | </StructureSection> | ||
[[Category: Fita, I | [[Category: Carpena, X]] | ||
[[Category: Garcia-Fernandez, Q | [[Category: Fita, I]] | ||
[[Category: Garreta, A | [[Category: Garcia-Fernandez, Q]] | ||
[[Category: Val-Moraes, S P | [[Category: Garreta, A]] | ||
[[Category: Val-Moraes, S P]] | |||
[[Category: Non-heme iron enzyme]] | [[Category: Non-heme iron enzyme]] | ||
[[Category: Oxidoreductase]] | [[Category: Oxidoreductase]] | ||
[[Category: Protein-phospholipid complex]] | [[Category: Protein-phospholipid complex]] | ||
Revision as of 11:28, 21 December 2014
Crystal Structure of a Phospholipid-Lipoxygenase Complex from Pseudomonas aeruginosa at 2.0 A (C2221)
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