4hph: Difference between revisions
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==The crystal structure of isomaltulose synthase mutant E295Q from Erwinia rhapontici NX5 in complex with its natural substrate sucrose== | |||
<StructureSection load='4hph' size='340' side='right' caption='[[4hph]], [[Resolution|resolution]] 1.70Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4hph]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Erwinia_rhapontici Erwinia rhapontici]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HPH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HPH FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SUC:SUCROSE'>SUC</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4how|4how]], [[4hox|4hox]], [[4hoz|4hoz]], [[4hp5|4hp5]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PalI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=55212 Erwinia rhapontici])</td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Isomaltulose_synthase Isomaltulose synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.99.11 5.4.99.11] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hph FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hph OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hph RCSB], [http://www.ebi.ac.uk/pdbsum/4hph PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Sucrose isomerase NX-5 from Erwiniarhapontici efficiently catalyzes the isomerization of sucrose to isomaltulose (main product) and trehalulose (by-product). To investigate the molecular mechanism controlling sucrose isomer formation, we determined the crystal structures of native NX-5 and its mutant complexes E295Q/sucrose and D241A/glucose at 1.70 A, 1.70 A and 2.00 A, respectively. The overall structure and active site architecture of NX-5 resemble those of other reported sucrose isomerases. Strikingly, the substrate binding mode of NX-5 is also similar to that of trehalulose synthase from Pseudomonasmesoacidophila MX-45 (MutB). Detailed structural analysis revealed the catalytic RXDRX motif and the adjacent 10-residue loop of NX-5 and isomaltulose synthase PalI from Klebsiella sp. LX3 adopt a distinct orientation from those of trehalulose synthases. Mutations of the loop region of NX-5 resulted in significant changes of the product ratio between isomaltulose and trehalulose. The molecular dynamics simulation data supported the product specificity of NX-5 towards isomaltulose and the role of the loop(330-339) in NX-5 catalysis. This work should prove useful for the engineering of sucrose isomerase for industrial carbohydrate biotransformations. | |||
The Structural Basis of Erwinia rhapontici Isomaltulose Synthase.,Xu Z, Li S, Li J, Li Y, Feng X, Wang R, Xu H, Zhou J PLoS One. 2013 Sep 19;8(9):e74788. doi: 10.1371/journal.pone.0074788. PMID:24069347<ref>PMID:24069347</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Erwinia rhapontici]] | [[Category: Erwinia rhapontici]] | ||
[[Category: Isomaltulose synthase]] | [[Category: Isomaltulose synthase]] | ||
[[Category: Li, S | [[Category: Li, S]] | ||
[[Category: Xu, H | [[Category: Xu, H]] | ||
[[Category: Xu, Z | [[Category: Xu, Z]] | ||
[[Category: Zhou, J | [[Category: Zhou, J]] | ||
[[Category: Calcium binding]] | [[Category: Calcium binding]] | ||
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
[[Category: Isomerase]] | [[Category: Isomerase]] | ||
[[Category: Tim barrel]] | [[Category: Tim barrel]] | ||
Revision as of 12:28, 21 December 2014
The crystal structure of isomaltulose synthase mutant E295Q from Erwinia rhapontici NX5 in complex with its natural substrate sucrose
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