4igb: Difference between revisions

Line 1:

~~{{STRUCTURE_4igb~~| ~~PDB~~=4igb | ~~SCENE~~= }}

==Crystal structure of the N-terminal domain of the Streptococcus gordonii adhesin Sgo0707==

===~~Crystal~~ structure of the N-terminal domain of the ~~Streptococcus~~ gordonii adhesin ~~Sgo0707===~~

~~{{ABSTRACT_PUBMED_23691093}}~~

== Structural highlights ==

<table><tr><td colspan='2'>[[4igb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_gordonii_str._challis Streptococcus gordonii str. challis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IGB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IGB FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>

<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SGO_0707 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29390 Streptococcus gordonii str. Challis])</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4igb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4igb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4igb RCSB], [http://www.ebi.ac.uk/pdbsum/4igb PDBsum]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

The commensal Streptococcus gordonii expresses numerous surface adhesins with which it interacts with other microorganisms, host cells and salivary proteins to initiate dental plaque formation. However, this Gram-positive bacterium can also spread to non-oral sites such as the heart valves and cause infective endocarditis. One of its surface adhesins, Sgo0707, is a large protein composed of a non-repetitive N-terminal region followed by several C-terminal repeat domains and a cell wall sorting motif. Here we present the crystal structure of the Sgo0707 N-terminal domains, refined to 2.1 A resolution. The model consists of two domains, N1 and N2. The largest domain, N1, comprises a putative binding cleft with a single cysteine located in its centre and exhibits an unexpected structural similarity to the variable domains of the streptococcal Antigen I/II adhesins. The N2-domain has an IgG-like fold commonly found among Gram-positive surface adhesins. Binding studies performed on S. gordonii wild-type and a Sgo0707 deficient mutant show that the Sgo0707 adhesin is involved in binding to type-1 collagen and to oral keratinocytes.

~~==About this Structure==~~

Structural and Functional Analysis of the N-terminal Domain of the Streptococcus gordonii Adhesin Sgo0707.,Nylander A, Svensater G, Senadheera DB, Cvitkovitch DG, Davies JR, Persson K PLoS One. 2013 May 17;8(5):e63768. doi: 10.1371/journal.pone.0063768. Print 2013. PMID:23691093<ref>PMID:23691093</ref>

~~[[4igb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_gordonii_str._challis~~ Streptococcus gordonii ~~str~~. ~~challis]~~. ~~Full crystallographic information is available from [http~~:/~~/oca~~.~~weizmann~~.ac.~~il/oca-bin~~/~~ocashort?id=4IGB OCA].~~

==~~Reference~~==

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

~~<ref group="xtra">PMID:023691093</ref>~~<references ~~group="xtra"~~/><~~references~~/>

</div>

== References ==

__TOC__

</StructureSection>

[[Category: Streptococcus gordonii str. challis]]

[[Category: Cvitkovitch, D G.]]

[[Category: Cvitkovitch, D G]]

[[Category: Davies, J R.]]

[[Category: Davies, J R]]

[[Category: Nylander, A.]]

[[Category: Nylander, A]]

[[Category: Persson, K.]]

[[Category: Persson, K]]

[[Category: Senadheera, D B.]]

[[Category: Senadheera, D B]]

[[Category: Svensater, G.]]

[[Category: Svensater, G]]

[[Category: Adhesin]]

[[Category: Beta-sandwich fold]]

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-{{STRUCTURE_4igb|  PDB=4igb  |  SCENE=  }}
+==Crystal structure of the N-terminal domain of the Streptococcus gordonii adhesin Sgo0707==
-===Crystal structure of the N-terminal domain of the Streptococcus gordonii adhesin Sgo0707===
+<StructureSection load='4igb' size='340' side='right' caption='[[4igb]], [[Resolution|resolution]] 2.09&Aring;' scene=''>
-{{ABSTRACT_PUBMED_23691093}}
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4igb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_gordonii_str._challis Streptococcus gordonii str. challis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IGB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IGB FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SGO_0707 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29390 Streptococcus gordonii str. Challis])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4igb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4igb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4igb RCSB], [http://www.ebi.ac.uk/pdbsum/4igb PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The commensal Streptococcus gordonii expresses numerous surface adhesins with which it interacts with other microorganisms, host cells and salivary proteins to initiate dental plaque formation. However, this Gram-positive bacterium can also spread to non-oral sites such as the heart valves and cause infective endocarditis. One of its surface adhesins, Sgo0707, is a large protein composed of a non-repetitive N-terminal region followed by several C-terminal repeat domains and a cell wall sorting motif. Here we present the crystal structure of the Sgo0707 N-terminal domains, refined to 2.1 A resolution. The model consists of two domains, N1 and N2. The largest domain, N1, comprises a putative binding cleft with a single cysteine located in its centre and exhibits an unexpected structural similarity to the variable domains of the streptococcal Antigen I/II adhesins. The N2-domain has an IgG-like fold commonly found among Gram-positive surface adhesins. Binding studies performed on S. gordonii wild-type and a Sgo0707 deficient mutant show that the Sgo0707 adhesin is involved in binding to type-1 collagen and to oral keratinocytes.
-==About this Structure==
+Structural and Functional Analysis of the N-terminal Domain of the Streptococcus gordonii Adhesin Sgo0707.,Nylander A, Svensater G, Senadheera DB, Cvitkovitch DG, Davies JR, Persson K PLoS One. 2013 May 17;8(5):e63768. doi: 10.1371/journal.pone.0063768. Print 2013. PMID:23691093<ref>PMID:23691093</ref>
-[[4igb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_gordonii_str._challis Streptococcus gordonii str. challis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IGB OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<ref group="xtra">PMID:023691093</ref><references group="xtra"/><references/>
+</div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Streptococcus gordonii str. challis]]
-[[Category: Cvitkovitch, D G.]]
+[[Category: Cvitkovitch, D G]]
-[[Category: Davies, J R.]]
+[[Category: Davies, J R]]
-[[Category: Nylander, A.]]
+[[Category: Nylander, A]]
-[[Category: Persson, K.]]
+[[Category: Persson, K]]
-[[Category: Senadheera, D B.]]
+[[Category: Senadheera, D B]]
-[[Category: Svensater, G.]]
+[[Category: Svensater, G]]
 [[Category: Adhesin]]
 [[Category: Beta-sandwich fold]]