4kki: Difference between revisions
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==Crystal Structure of Haptocorrin in Complex with CNCbl== | |||
<StructureSection load='4kki' size='340' side='right' caption='[[4kki]], [[Resolution|resolution]] 2.35Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4kki]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KKI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KKI FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CNC:CO-CYANOCOBALAMIN'>CNC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4kkj|4kkj]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TC1, TCN1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kki FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kki OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4kki RCSB], [http://www.ebi.ac.uk/pdbsum/4kki PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Cobalamin (Cbl; vitamin B12) is an essential micronutrient synthesized only by bacteria. Mammals have developed a sophisticated uptake system to capture the vitamin from the diet. Cbl transport is mediated by three transport proteins: transcobalamin, intrinsic factor, and haptocorrin (HC). All three proteins have a similar overall structure but a different selectivity for corrinoids. Here, we present the crystal structures of human HC in complex with cyanocobalamin and cobinamide at 2.35 and 3.0 A resolution, respectively. The structures reveal that many of the interactions with the corrin ring are conserved among the human Cbl transporters. However, the non-conserved residues Asn-120, Arg-357, and Asn-373 form distinct interactions allowing for stabilization of corrinoids other than Cbl. A central binding motif forms interactions with the e- and f-side chains of the corrin ring and is conserved in corrinoid-binding proteins of other species. In addition, the alpha- and beta-domains of HC form several unique interdomain contacts and have a higher shape complementarity than those of intrinsic factor and transcobalamin. The stabilization of ligands by all of these interactions is reflected in higher melting temperatures of the protein-ligand complexes. Our structural analysis offers fundamental insights into the unique binding behavior of HC and completes the picture of Cbl interaction with its three transport proteins. | |||
Structural basis for universal corrinoid recognition by the cobalamin transport protein haptocorrin.,Furger E, Frei DC, Schibli R, Fischer E, Prota AE J Biol Chem. 2013 Aug 30;288(35):25466-76. doi: 10.1074/jbc.M113.483271. Epub, 2013 Jul 11. PMID:23846701<ref>PMID:23846701</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
== | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Fischer, E | [[Category: Fischer, E]] | ||
[[Category: Frei, D C | [[Category: Frei, D C]] | ||
[[Category: Furger, E | [[Category: Furger, E]] | ||
[[Category: Prota, A E | [[Category: Prota, A E]] | ||
[[Category: Schibli, R | [[Category: Schibli, R]] | ||
[[Category: Alpha6-alpha6 helical barrel]] | [[Category: Alpha6-alpha6 helical barrel]] | ||
[[Category: Cobalamin transport protein]] | [[Category: Cobalamin transport protein]] | ||
[[Category: Transport protein]] | [[Category: Transport protein]] | ||
Revision as of 14:38, 21 December 2014
Crystal Structure of Haptocorrin in Complex with CNCbl
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