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{{STRUCTURE_4m4w|  PDB=4m4w  |  SCENE=  }}
==Mechanistic implications for the bacterial primosome assembly of the structure of a helicase-helicase loader complex==
===Mechanistic implications for the bacterial primosome assembly of the structure of a helicase-helicase loader complex===
<StructureSection load='4m4w' size='340' side='right' caption='[[4m4w]], [[Resolution|resolution]] 6.10&Aring;' scene=''>
{{ABSTRACT_PUBMED_24048025}}
== Structural highlights ==
<table><tr><td colspan='2'>[[4m4w]] is a 15 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168] and [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M4W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4M4W FirstGlance]. <br>
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">dnaB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1422 Geobacillus stearothermophilus]), dnaG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1422 Geobacillus stearothermophilus]), BSU28980, dnaI, ytxA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 Bacillus subtilis subsp. subtilis str. 168])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m4w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m4w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4m4w RCSB], [http://www.ebi.ac.uk/pdbsum/4m4w PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
During the assembly of the bacterial loader-dependent primosome, helicase loader proteins bind to the hexameric helicase ring, deliver it onto the oriC DNA and then dissociate from the complex. Here, to provide a better understanding of this key process, we report the crystal structure of the ~570-kDa prepriming complex between the Bacillus subtilis loader protein and the Bacillus stearothermophilus helicase, as well as the helicase-binding domain of primase with a molar ratio of 6:6:3 at 7.5 A resolution. The overall architecture of the complex exhibits a three-layered ring conformation. Moreover, the structure combined with the proposed model suggests that the shift from the 'open-ring' to the 'open-spiral' and then the 'closed-spiral' state of the helicase ring due to the binding of single-stranded DNA may be the cause of the loader release.


==Function==
Structure of a helicase-helicase loader complex reveals insights into the mechanism of bacterial primosome assembly.,Liu B, Eliason WK, Steitz TA Nat Commun. 2013;4:2495. doi: 10.1038/ncomms3495. PMID:24048025<ref>PMID:24048025</ref>
[[http://www.uniprot.org/uniprot/DNAI_BACSU DNAI_BACSU]] Probably involved in DNA replication. [[http://www.uniprot.org/uniprot/PRIM_GEOSE PRIM_GEOSE]] DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[4m4w]] is a 15 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168] and [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M4W OCA].
</div>


==Reference==
==See Also==
<ref group="xtra">PMID:024048025</ref><references group="xtra"/><references/>
*[[RNA polymerase|RNA polymerase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacillus subtilis subsp. subtilis str. 168]]
[[Category: Bacillus subtilis subsp. subtilis str. 168]]
[[Category: Geobacillus stearothermophilus]]
[[Category: Geobacillus stearothermophilus]]
[[Category: Eliason, W K.]]
[[Category: Eliason, W K]]
[[Category: Liu, B.]]
[[Category: Liu, B]]
[[Category: Steitz, T A.]]
[[Category: Steitz, T A]]
[[Category: Dna replication]]
[[Category: Dna replication]]
[[Category: Dnab]]
[[Category: Dnab]]
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