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{{STRUCTURE_4n71|  PDB=4n71  |  SCENE=  }}
==X-Ray Crystal Structure of 2-amino-1-hydroxyethylphosphonate-bound PhnZ==
===X-Ray Crystal Structure of 2-amino-1-hydroxyethylphosphonate-bound PhnZ===
<StructureSection load='4n71' size='340' side='right' caption='[[4n71]], [[Resolution|resolution]] 2.98&Aring;' scene=''>
{{ABSTRACT_PUBMED_24198335}}
== Structural highlights ==
<table><tr><td colspan='2'>[[4n71]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Uncultured_bacterium_hf130_aepn_1 Uncultured bacterium hf130_aepn_1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N71 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4N71 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=ODV:[(1R)-2-AMINO-1-HYDROXYETHYL]PHOSPHONIC+ACID'>ODV</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4n6w|4n6w]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ACU83550, ALOHA_HF130_AEPn_1_06c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=663362 uncultured bacterium HF130_AEPn_1])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4n71 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n71 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4n71 RCSB], [http://www.ebi.ac.uk/pdbsum/4n71 PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The founding members of the HD-domain protein superfamily are phosphohydrolases, and newly discovered members are generally annotated as such. However, myo-inositol oxygenase (MIOX) exemplifies a second, very different function that has evolved within the common scaffold of this superfamily. A recently discovered HD protein, PhnZ, catalyzes conversion of 2-amino-1-hydroxyethylphosphonate to glycine and phosphate, culminating a bacterial pathway for the utilization of environmentally abundant 2-aminoethylphosphonate. Using Mossbauer and EPR spectroscopies, X-ray crystallography, and activity measurements, we show here that, like MIOX, PhnZ employs a mixed-valent Fe(II)/Fe(III) cofactor for the O2-dependent oxidative cleavage of its substrate. Phylogenetic analysis suggests that many more HD proteins may catalyze yet-unknown oxygenation reactions using this hitherto exceptional Fe(II)/Fe(III) cofactor. The results demonstrate that the catalytic repertoire of the HD superfamily extends well beyond phosphohydrolysis and suggest that the mechanism used by MIOX and PhnZ may be a common strategy for oxidative C-X bond cleavage.


==About this Structure==
Organophosphonate-degrading PhnZ reveals an emerging family of HD domain mixed-valent diiron oxygenases.,Worsdorfer B, Lingaraju M, Yennawar NH, Boal AK, Krebs C, Bollinger JM Jr, Pandelia ME Proc Natl Acad Sci U S A. 2013 Nov 19;110(47):18874-9. doi:, 10.1073/pnas.1315927110. Epub 2013 Nov 6. PMID:24198335<ref>PMID:24198335</ref>
[[4n71]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Uncultured_bacterium_hf130_aepn_1 Uncultured bacterium hf130_aepn_1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N71 OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
<ref group="xtra">PMID:024198335</ref><references group="xtra"/><references/>
</div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Uncultured bacterium hf130_aepn_1]]
[[Category: Uncultured bacterium hf130_aepn_1]]
[[Category: Boal, A K.]]
[[Category: Boal, A K]]
[[Category: Jr, J M.Bollinger.]]
[[Category: Jr, J M.Bollinger]]
[[Category: Krebs, C.]]
[[Category: Krebs, C]]
[[Category: Lingaraju, M.]]
[[Category: Lingaraju, M]]
[[Category: Pandelia, M E.]]
[[Category: Pandelia, M E]]
[[Category: Woersdoerfer, B.]]
[[Category: Woersdoerfer, B]]
[[Category: Yennawar, N.]]
[[Category: Yennawar, N]]
[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
[[Category: Oxygenase]]
[[Category: Oxygenase]]
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