2bic: Difference between revisions
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==The solution structure of the recombinant elicitor protein PcF from the oomycete pathogen P. cactorum== | |||
<StructureSection load='2bic' size='340' side='right' caption='[[2bic]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2bic]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Phytophthora_cactorum Phytophthora cactorum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BIC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BIC FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bic OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2bic RCSB], [http://www.ebi.ac.uk/pdbsum/2bic PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The PcF protein from Phytophthora cactorum is the first member of the "PcF toxin family" from the plant pathogens Phytophthora spp. It is able to induce withering in tomato and strawberry leaves. The lack of sequence similarity with other proteins hampers the identification of the molecular mechanisms responsible for its toxicity. Here, we show that the six cysteines form a disulphide pattern that is exclusive for PcF and essential for the protein withering activity. The NMR solution structure identifies a novel fold among protein effectors: a helix-loop-helix motif. The presence of a negatively charged surface suggests that it might act as a site of electrostatic interaction. Interestingly, a good fold match with Ole e 6, a plant protein with allergenic activity, highlighted the spatial superimposition of a stretch of identical residues. This finding suggests a possible biological activity based on molecular mimicry. | |||
Solution structure of the phytotoxic protein PcF: the first characterized member of the Phytophthora PcF toxin family.,Nicastro G, Orsomando G, Ferrari E, Manconi L, Desario F, Amici A, Naso A, Carpaneto A, Pertinhez TA, Ruggieri S, Spisni A Protein Sci. 2009 Aug;18(8):1786-91. PMID:19554629<ref>PMID:19554629</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Phytophthora cactorum]] | [[Category: Phytophthora cactorum]] | ||
[[Category: Desario, F | [[Category: Desario, F]] | ||
[[Category: Ferrari, E | [[Category: Ferrari, E]] | ||
[[Category: Manconi, L | [[Category: Manconi, L]] | ||
[[Category: Nicastro, G | [[Category: Nicastro, G]] | ||
[[Category: Orsomando, G | [[Category: Orsomando, G]] | ||
[[Category: Ruggieri, S | [[Category: Ruggieri, S]] | ||
[[Category: Spisni, A | [[Category: Spisni, A]] | ||
[[Category: Elicitin family]] | [[Category: Elicitin family]] | ||
[[Category: Oomycete plant pathogen]] | [[Category: Oomycete plant pathogen]] | ||
