2m39: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==The solution structure of 3',5'-LINKED 2'-O-(2-METHOXYETHYL)-RNA DUPLEX== | |||
<StructureSection load='2m39' size='340' side='right' caption='[[2m39]], [[NMR_Ensembles_of_Models | 9 NMR models]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2m39]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M39 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2M39 FirstGlance]. <br> | |||
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=A44:2-O-METHYOXYETHYL-ADENOSINE+5-MONOPHOSPHATE'>A44</scene>, <scene name='pdbligand=C5L:2-O-(2-METHOXYETHYL)-5-METHYLCYTIDINE+5-(DIHYDROGEN+PHOSPHATE)'>C5L</scene>, <scene name='pdbligand=G48:2-O-METHYOXYETHYL-GUANOSINE-5-MONOPHOSPHATE'>G48</scene>, <scene name='pdbligand=T39:2-O-METHOXYETHYLENE+THYMIDINE+5-MONOPHOSPHATE'>T39</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2m39 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m39 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2m39 RCSB], [http://www.ebi.ac.uk/pdbsum/2m39 PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The solution-state structure of 2'-O-(2-methoxyethly) substituted dodecamer r(*CG*CGAA*U*U*CG*C)d(G), 2'-MOE RNA, with all cytosines and uracils methylated at the C5-position has been determined by NMR spectroscopy. The chemical modifications were used to improve the oligonucleotide's drug-like properties. The 2'-MOE group drives pseudorotational equilibrium of the ribofuranose moiety to the N-type conformation and supposedly results in structural preorganization leading to high affinity of a modified oligonucleotide towards its complementary biological target, improved pharmacokinetic and toxicological properties. The high melting temperature of the antiparallel duplex structure adopted by 2'-MOE RNA was explained through the formation of a stable A-form RNA consistent with effective base-pairing and stacking interactions. The comparison of the solution-state structure with the crystal structure of a non-methylated analogue shows an increase in the stacking at the base pair steps for the C5-methylated 2'-MOE RNA duplex. The MOE substituents adopt a well-defined structure in the minor groove with the predominant gauche conformations around the ethylene bond. | |||
NMR structure of 2'-O-(2-methoxyethyl) modified and C5-methylated RNA dodecamer duplex.,Plevnik M, Cevec M, Plavec J Biochimie. 2013 Dec;95(12):2385-91. doi: 10.1016/j.biochi.2013.08.025. Epub 2013 , Sep 3. PMID:24012551<ref>PMID:24012551</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
[[Category: Cevec, M | == References == | ||
[[Category: Plavec, J | <references/> | ||
[[Category: Plevnik, M | __TOC__ | ||
</StructureSection> | |||
[[Category: Cevec, M]] | |||
[[Category: Plavec, J]] | |||
[[Category: Plevnik, M]] | |||
[[Category: Duplex]] | [[Category: Duplex]] | ||
[[Category: Rna]] | [[Category: Rna]] | ||
Revision as of 18:13, 21 December 2014
The solution structure of 3',5'-LINKED 2'-O-(2-METHOXYETHYL)-RNA DUPLEX
| ||||||||||||
