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{{STRUCTURE_4mna|  PDB=4mna | SCENE= }}
==Crystal structure of the free FLS2 ectodomains==
===Crystal structure of the free FLS2 ectodomains===
<StructureSection load='4mna' size='340' side='right' caption='[[4mna]], [[Resolution|resolution]] 4.00&Aring;' scene=''>
{{ABSTRACT_PUBMED_24114786}}
== Structural highlights ==
<table><tr><td colspan='2'>[[4mna]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MNA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MNA FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4mn8|4mn8]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FLS2, At5g46330, MPL12.13, MPL12.8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mna FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mna OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mna RCSB], [http://www.ebi.ac.uk/pdbsum/4mna PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Flagellin perception in Arabidopsis is through recognition of its highly conserved N-terminal epitope (flg22) by flagellin-sensitive 2 (FLS2). Flg22 binding induces FLS2 heteromerization with BRASSINOSTEROID INSENSITIVE 1-associated kinase 1 (BAK1) and their reciprocal activation followed by plant immunity. Here, we report the crystal structure of FLS2 and BAK1 ectodomains complexed with flg22 at 3.06 angstroms. A conserved and a nonconserved site from the inner surface of the FLS2 solenoid recognize the C- and N-terminal segment of flg22, respectively, without oligomerization or conformational changes in the FLS2 ectodomain. Besides directly interacting with FLS2, BAK1 acts as a co-receptor by recognizing the C terminus of the FLS2-bound flg22. Our data reveal the molecular mechanisms underlying FLS2-BAK1 complex recognition of flg22 and provide insight into the immune receptor complex activation.


==Function==
Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex.,Sun Y, Li L, Macho AP, Han Z, Hu Z, Zipfel C, Zhou JM, Chai J Science. 2013 Nov 1;342(6158):624-8. doi: 10.1126/science.1243825. Epub 2013 Oct , 10. PMID:24114786<ref>PMID:24114786</ref>
[[http://www.uniprot.org/uniprot/FLS2_ARATH FLS2_ARATH]] Constitutes the pattern-recognition receptor (PPR) that determines the specific perception of flagellin (flg22), a potent elicitor of the defense response to pathogen-associated molecular patterns (PAMPs). Flagellin-binding to the receptor is the first step to initiate the innate immune MAP kinase signaling cascade (MEKK1, MKK4/MKK5 and MPK3/MPK6), resulting in enhanced resistance against pathogens. Binding to the effector AvrPto1 from Pseudomonas syringae blocks the downstream plant immune response.<ref>PMID:10911994</ref> <ref>PMID:11340188</ref> <ref>PMID:11875555</ref> <ref>PMID:15085136</ref> <ref>PMID:19062288</ref> <ref>PMID:16377758</ref>


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[4mna]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MNA OCA].
</div>
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:024114786</ref><references group="xtra"/><references/>
__TOC__
</StructureSection>
[[Category: Arath]]
[[Category: Non-specific serine/threonine protein kinase]]
[[Category: Non-specific serine/threonine protein kinase]]
[[Category: Chai, J.]]
[[Category: Chai, J]]
[[Category: Han, Z.]]
[[Category: Han, Z]]
[[Category: Sun, Y.]]
[[Category: Sun, Y]]
[[Category: Fls2]]
[[Category: Fls2]]
[[Category: Leucine-rich repeat]]
[[Category: Leucine-rich repeat]]
[[Category: Plant immunity]]
[[Category: Plant immunity]]
[[Category: Transferase]]
[[Category: Transferase]]

Revision as of 18:21, 21 December 2014

Crystal structure of the free FLS2 ectodomains

4mna, resolution 4.00Å

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