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{{STRUCTURE_3t72|  PDB=3t72  |  SCENE=  }}
==PhoB(E)-Sigma70(4)-(RNAP-Betha-flap-tip-helix)-DNA Transcription Activation Sub-Complex==
===PhoB(E)-Sigma70(4)-(RNAP-Betha-flap-tip-helix)-DNA Transcription Activation Sub-Complex===
<StructureSection load='3t72' size='340' side='right' caption='[[3t72]], [[Resolution|resolution]] 4.33&Aring;' scene=''>
{{ABSTRACT_PUBMED_21829166}}
== Structural highlights ==
<table><tr><td colspan='2'>[[3t72]] is a 50 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T72 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T72 FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">phoB, b0399, JW0389 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI]), rpoD, alt, b3067, JW3039, EKO11_4334, rpoB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t72 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t72 RCSB], [http://www.ebi.ac.uk/pdbsum/3t72 PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
PhoB is a two-component response regulator that activates transcription by interacting with the sigma(70) subunit of the E. coli RNA polymerase in promoters in which the -35 sigma(70)-recognition element is replaced by the pho box. The crystal structure of a transcription initiation subcomplex that includes the sigma(4) domain of sigma(70) fused with the RNA polymerase beta subunit flap tip helix, the PhoB effector domain and the pho box DNA reveals how sigma(4) recognizes the upstream pho box repeat. As with the -35 element, sigma(4) achieves this recognition through the N-terminal portion of its DNA recognition helix, but contact with the DNA major groove is less extensive. Unexpectedly, the same recognition helix contacts the transactivation loop and helices alpha2 and alpha3 of PhoB. This result shows a simple and elegant mechanism for polymerase recruitment to pho box promoters in which the lost -35 element contacts are compensated by new ones with the activator. In addition, sigma(4) is reoriented, thereby suggesting a remodelling mechanism for transcription initiation.


==Function==
The structure of a transcription activation subcomplex reveals how sigma(70) is recruited to PhoB promoters.,Blanco AG, Canals A, Bernues J, Sola M, Coll M EMBO J. 2011 Aug 9;30(18):3776-85. doi: 10.1038/emboj.2011.271. PMID:21829166<ref>PMID:21829166</ref>
[[http://www.uniprot.org/uniprot/RPOD_ECOLI RPOD_ECOLI]] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium. [[http://www.uniprot.org/uniprot/PHOB_ECOLI PHOB_ECOLI]] This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by PhoB and PhoR when phosphate is limited.  


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[3t72]] is a 50 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T72 OCA].
</div>


==See Also==
==See Also==
*[[RNA polymerase|RNA polymerase]]
*[[RNA polymerase|RNA polymerase]]
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:021829166</ref><references group="xtra"/><references/>
__TOC__
</StructureSection>
[[Category: DNA-directed RNA polymerase]]
[[Category: DNA-directed RNA polymerase]]
[[Category: Ecoli]]
[[Category: Ecoli]]
[[Category: Bernues, J.]]
[[Category: Bernues, J]]
[[Category: Blanco, A G.]]
[[Category: Blanco, A G]]
[[Category: Canals, A.]]
[[Category: Canals, A]]
[[Category: Coll, M.]]
[[Category: Coll, M]]
[[Category: Sola, M.]]
[[Category: Sola, M]]
[[Category: Dna-binding]]
[[Category: Dna-binding]]
[[Category: Transcription activation]]
[[Category: Transcription activation]]
[[Category: Transcription-dna complex]]
[[Category: Transcription-dna complex]]
[[Category: Winged-helix motif]]
[[Category: Winged-helix motif]]
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