2lt5: Difference between revisions
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[[ | ==Zymogen-FLG of the onconase== | ||
<StructureSection load='2lt5' size='340' side='right' caption='[[2lt5]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2lt5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rana_pipiens Rana pipiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LT5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LT5 FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lt5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lt5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2lt5 RCSB], [http://www.ebi.ac.uk/pdbsum/2lt5 PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Onconase((R)) FL-G zymogen is a 120 residue protein produced by circular permutation of the native Onconase((R)) sequence. In this construction, the wild type N- and C-termini are linked by a 16 residue segment and new N- and C-termini are generated at wild type positions R73 and S72. This novel segment linking the native N- and C-termini is designed to obstruct Onconase's((R)) active site and encloses a cleavage site for the HIV-1 protease. As a first step towards the resolution of its 3D structure and the study of its structure-function relationships, we report here the nearly complete NMR (1)H, (13)C and (15)N resonance chemical shift assignments at pH 5.2 and 35 degrees C (BMRB deposit no 17973). The results presented here clearly show that the structure of the wild type Onconase((R)) is conserved in the FL-G zymogen. | |||
(1)H, (13)C and (15)N resonance assignments of the Onconase FL-G zymogen.,Serrano S, Callis M, Vilanova M, Benito A, Laurents DV, Ribo M, Bruix M Biomol NMR Assign. 2012 Mar 4. PMID:22392335<ref>PMID:22392335</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
== | |||
< | |||
[[Category: Rana pipiens]] | [[Category: Rana pipiens]] | ||
[[Category: Bruix, M | [[Category: Bruix, M]] | ||
[[Category: Callis, M | [[Category: Callis, M]] | ||
[[Category: Laurents, D V | [[Category: Laurents, D V]] | ||
[[Category: Ribo, M | [[Category: Ribo, M]] | ||
[[Category: Serrano, S | [[Category: Serrano, S]] | ||
[[Category: Vilanova, M | [[Category: Vilanova, M]] | ||
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
[[Category: Ribonuclease]] | [[Category: Ribonuclease]] | ||
