1t8b: Difference between revisions
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'''Crystal structure of refolded PHOU-like protein (gi 2983430) from Aquifex aeolicus''' | {{Structure | ||
|PDB= 1t8b |SIZE=350|CAPTION= <scene name='initialview01'>1t8b</scene>, resolution 3.23Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= PHOU, AQ_906 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus]) | |||
}} | |||
'''Crystal structure of refolded PHOU-like protein (gi 2983430) from Aquifex aeolicus''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1T8B is a [ | 1T8B is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8B OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of the "PhoU-like" phosphate uptake regulator from Aquifex aeolicus., Oganesyan V, Oganesyan N, Adams PD, Jancarik J, Yokota HA, Kim R, Kim SH, J Bacteriol. 2005 Jun;187(12):4238-44. PMID:[http:// | Crystal structure of the "PhoU-like" phosphate uptake regulator from Aquifex aeolicus., Oganesyan V, Oganesyan N, Adams PD, Jancarik J, Yokota HA, Kim R, Kim SH, J Bacteriol. 2005 Jun;187(12):4238-44. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15937186 15937186] | ||
[[Category: Aquifex aeolicus]] | [[Category: Aquifex aeolicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Oganesyan, N.]] | [[Category: Oganesyan, N.]] | ||
[[Category: Oganesyan, V.]] | [[Category: Oganesyan, V.]] | ||
[[Category: alpha-helical protein consisting of two 3-helix | [[Category: alpha-helical protein consisting of two 3-helix bundle]] | ||
[[Category: berkeley structural genomics center]] | [[Category: berkeley structural genomics center]] | ||
[[Category: bsgc structure funded by nih]] | [[Category: bsgc structure funded by nih]] | ||
[[Category: protein structure initiative]] | [[Category: protein structure initiative]] | ||
[[Category: psi]] | [[Category: psi]] | ||
[[Category: structural | [[Category: structural genomic]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:15:08 2008'' | ||
Revision as of 12:15, 20 March 2008
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| 1t8b, resolution 3.23Å | |||||||||||||
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| Gene: | PHOU, AQ_906 (Aquifex aeolicus) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of refolded PHOU-like protein (gi 2983430) from Aquifex aeolicus
Overview
The phoU gene of Aquifex aeolicus encodes a protein called PHOU_AQUAE with sequence similarity to the PhoU protein of Escherichia coli. Despite the fact that there is a large number of family members (more than 300) attributed to almost all known bacteria and despite PHOU_AQUAE's association with the regulation of genes for phosphate metabolism, the nature of its regulatory function is not well understood. Nearly one-half of these PhoU-like proteins, including both PHOU_AQUAE and the one from E. coli, form a subfamily with an apparent dimer structure of two PhoU domains on the basis of their amino acid sequence. The crystal structure of PHOU_AQUAE (a 221-amino-acid protein) reveals two similar coiled-coil PhoU domains, each forming a three-helix bundle. The structures of PHOU_AQUAE proteins from both a soluble fraction and refolded inclusion bodies (at resolutions of 2.8 and 3.2A, respectively) showed no significant differences. The folds of the PhoU domain and Bag domains (for a class of cofactors of the eukaryotic chaperone Hsp70 family) are similar. Accordingly, we propose that gene regulation by PhoU may occur by association of PHOU_AQUAE with the ATPase domain of the histidine kinase PhoR, promoting release of its substrate PhoB. Other proteins that share the PhoU domain fold include the coiled-coil domains of the STAT protein, the ribosome-recycling factor, and structural proteins like spectrin.
About this Structure
1T8B is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.
Reference
Crystal structure of the "PhoU-like" phosphate uptake regulator from Aquifex aeolicus., Oganesyan V, Oganesyan N, Adams PD, Jancarik J, Yokota HA, Kim R, Kim SH, J Bacteriol. 2005 Jun;187(12):4238-44. PMID:15937186
Page seeded by OCA on Thu Mar 20 14:15:08 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Aquifex aeolicus
- Single protein
- Adams, P D.
- BSGC, Berkeley Structural Genomics Center.
- Jancarik, J.
- Kim, R.
- Kim, S H.
- Oganesyan, N.
- Oganesyan, V.
- Alpha-helical protein consisting of two 3-helix bundle
- Berkeley structural genomics center
- Bsgc structure funded by nih
- Protein structure initiative
- Psi
- Structural genomic
