1ca5: Difference between revisions

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{{STRUCTURE_1ca5|  PDB=1ca5  |  SCENE=  }}
==INTERCALATION SITE OF HYPERTHERMOPHILE CHROMOSOMAL PROTEIN SSO7D/SAC7D BOUND TO DNA==
===INTERCALATION SITE OF HYPERTHERMOPHILE CHROMOSOMAL PROTEIN SSO7D/SAC7D BOUND TO DNA===
<StructureSection load='1ca5' size='340' side='right' caption='[[1ca5]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
{{ABSTRACT_PUBMED_11031116}}
== Structural highlights ==
<table><tr><td colspan='2'>[[1ca5]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CA5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CA5 FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ca5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ca5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ca5 RCSB], [http://www.ebi.ac.uk/pdbsum/1ca5 PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Sso7d and Sac7d are two small chromatin proteins from the hyperthermophilic archaeabacterium Sulfolobus solfataricus and Sulfolobus acidocaldarius, respectively. The crystal structures of Sso7d-GTGATCGC, Sac7d-GTGATCGC and Sac7d-GTGATCAC have been determined and refined at 1.45 A, 2.2 A and 2.2 A, respectively, to investigate the DNA binding property of Sso7d/Sac7d in the presence of a T-G mismatch base-pair. Detailed structural analysis revealed that the intercalation site includes the T-G mismatch base-pair and Sso7d/Sac7d bind to that mismatch base-pair in a manner similar to regular DNA. In the Sso7d-GTGATCGC complex, a new inter-strand hydrogen bond between T2O4 and C14N4 is formed and well-order bridging water molecules are found. The results suggest that the less stable DNA stacking site involving a T-G mismatch may be a preferred site for protein side-chain intercalation.


==Function==
Crystal structures of the chromosomal proteins Sso7d/Sac7d bound to DNA containing T-G mismatched base-pairs.,Su S, Gao YG, Robinson H, Liaw YC, Edmondson SP, Shriver JW, Wang AH J Mol Biol. 2000 Oct 27;303(3):395-403. PMID:11031116<ref>PMID:11031116</ref>
[[http://www.uniprot.org/uniprot/DN71_SULAC DN71_SULAC]] Constrain negative DNA supercoils; may be involved in maintaining the integrity of their genome at high temperature.  


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[1ca5]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CA5 OCA].
</div>
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:011031116</ref><references group="xtra"/><references/>
__TOC__
</StructureSection>
[[Category: Sulfolobus acidocaldarius]]
[[Category: Sulfolobus acidocaldarius]]
[[Category: Gao, Y G.]]
[[Category: Gao, Y G]]
[[Category: Robinson, H.]]
[[Category: Robinson, H]]
[[Category: Shriver, J W.]]
[[Category: Shriver, J W]]
[[Category: Su, S.]]
[[Category: Su, S]]
[[Category: Wang, A H.J.]]
[[Category: Wang, A H.J]]
[[Category: Chromosomal protein]]
[[Category: Chromosomal protein]]
[[Category: Dna binding]]
[[Category: Dna binding]]
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