1ql5: Difference between revisions
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==DNA DECAMER DUPLEX CONTAINING T5-T6 PHOTOADDUCT== | |||
=== | <StructureSection load='1ql5' size='340' side='right' caption='[[1ql5]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1ql5]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QL5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QL5 FirstGlance]. <br> | |||
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PBT:[3-HYDROXY-5-(5-METHYL-2,4-DIOXOTETRAHYDRO-1(2H)-PYRIMIDINYL)TETRAHYDRO-2-FURANYL]METHYL+DIHYDROGEN+PHOSPHATE'>PBT</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ql5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ql5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ql5 RCSB], [http://www.ebi.ac.uk/pdbsum/1ql5 PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The cis - syn dimer is the major DNA photoproduct produced by UV irradiation. In order to determine the origin of the mutagenic property of the cis - syn dimer, we used NMR restraints and molecular dynamics to determine the solution structure of a DNA decamer duplex containing a wobble pair between the 3'-T of the cis - syn dimer and the opposite T residue (CS/TA duplex). The solution structure of the CS/TA duplex revealed that the 3'-T x T base pair of the cis - syn dimer had base pair geometry that was significantly different from the canonical Watson-Crick base pair and caused destabilization and conformational distortion of its 3'-region. However, a 3'-T x A base pair at the cis - syn dimer within this related DNA decamer maintains the normal Watson-Crick base pair geometry and causes little distortion in the conformation of its 3'-side. Our results show that in spite of its stable hydrogen bonding, the insertion of a T residue opposite the 3'-T of the cis - syn dimer is inhibited by structural distortion caused by the 3'-T x T base pair. This may explain why the frequency of the 3'-T-->A transversion, which is the major mutation produced by the cis - syn dimer, is only 4%. | |||
Solution structure of the DNA decamer duplex containing a 3'-T x T basepair of the cis-syn cyclobutane pyrimidine dimer: implication for the mutagenic property of the cis-syn dimer.,Lee JH, Choi YJ, Choi BS Nucleic Acids Res. 2000 Apr 15;28(8):1794-801. PMID:10734199<ref>PMID:10734199</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
[[Category: Choi, B S | == References == | ||
[[Category: Hwang, G S | <references/> | ||
[[Category: Lee, J H | __TOC__ | ||
</StructureSection> | |||
[[Category: Choi, B S]] | |||
[[Category: Hwang, G S]] | |||
[[Category: Lee, J H]] | |||
[[Category: Deoxyribonucleic acid]] | [[Category: Deoxyribonucleic acid]] | ||
[[Category: Dna]] | [[Category: Dna]] | ||
[[Category: Dna photoproduct]] | [[Category: Dna photoproduct]] | ||
[[Category: Mutagenesis]] | [[Category: Mutagenesis]] | ||
