1f5u: Difference between revisions

Line 1:

[[~~Image~~:1f5u.~~png|left|200px~~]]

==SOLUTION STRUCTURE OF THE KISSING DIMER OF H3 GACG STEM-LOOP IN THE 5'-END DIMERIZATION SIGNAL OF MOLONEY MURINE LEUKEMIA VIRUS GENOMIC RNA==

== Structural highlights ==

<table><tr><td colspan='2'>[[1f5u]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F5U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1F5U FirstGlance]. <br>

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f5u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1f5u RCSB], [http://www.ebi.ac.uk/pdbsum/1f5u PDBsum]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

The dimerization of viral RNA through noncovalent interactions at their 5' ends is a key step in the life cycle of retroviruses. In Moloney murine leukemia virus, three stem-loops are important in this process. One is a self-complementary tetraloop (H1), but the other two stem-loops (H2, H3) contain highly conserved GACG tetraloops that are not self-complementary sequences. Using two-dimensional NMR, we determined the structure of the H3 stem-loop. Surprisingly, it forms a stable, homodimeric kissing complex through only two intermolecular G small middle dotC base pairs. Cross-strand interactions of the adenines adjacent to the intermolecular G small middle dotC base pairs, plus unusual strong electrostatic interactions around the base pairs, contribute to the unexpected stability. This structure shows how even stem-loops without self-complementary sequences can facilitate the intermolecular recognition between two identical RNAs, and thus initiate dimerization and encapsidation of retroviral RNAs.

~~{{STRUCTURE_1f5u| PDB=1f5u | SCENE= }}~~

A retroviral RNA kissing complex containing only two G.C base pairs.,Kim CH, Tinoco I Jr Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9396-401. PMID:10931958<ref>PMID:10931958</ref>

~~===SOLUTION STRUCTURE OF THE KISSING DIMER OF H3 GACG STEM-LOOP IN THE 5'-END DIMERIZATION SIGNAL OF MOLONEY MURINE LEUKEMIA VIRUS GENOMIC RNA===~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

== References ==

~~==About this Structure==~~

~~[[1f5u]] is~~ a ~~2 chain structure. Full experimental information is available from [http://oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=1F5U OCA].~~

__TOC__

</StructureSection>

==~~Reference~~==

[[Category: Kim, C H]]

<~~ref group="xtra">PMID:010931958<~~/~~ref~~><~~references group="xtra"~~/>

[[Category: Tinoco, I]]

[[Category: Kim, C H.]]

[[Category: Tinoco, I.]]

[[Category: Dimerization]]

[[Category: Kissing dimer]]

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-[[Image:1f5u.png|left|200px]]
+==SOLUTION STRUCTURE OF THE KISSING DIMER OF H3 GACG STEM-LOOP IN THE 5'-END DIMERIZATION SIGNAL OF MOLONEY MURINE LEUKEMIA VIRUS GENOMIC RNA==
+<StructureSection load='1f5u' size='340' side='right' caption='[[1f5u]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1f5u]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F5U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1F5U FirstGlance]. <br>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f5u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1f5u RCSB], [http://www.ebi.ac.uk/pdbsum/1f5u PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The dimerization of viral RNA through noncovalent interactions at their 5' ends is a key step in the life cycle of retroviruses. In Moloney murine leukemia virus, three stem-loops are important in this process. One is a self-complementary tetraloop (H1), but the other two stem-loops (H2, H3) contain highly conserved GACG tetraloops that are not self-complementary sequences. Using two-dimensional NMR, we determined the structure of the H3 stem-loop. Surprisingly, it forms a stable, homodimeric kissing complex through only two intermolecular G small middle dotC base pairs. Cross-strand interactions of the adenines adjacent to the intermolecular G small middle dotC base pairs, plus unusual strong electrostatic interactions around the base pairs, contribute to the unexpected stability. This structure shows how even stem-loops without self-complementary sequences can facilitate the intermolecular recognition between two identical RNAs, and thus initiate dimerization and encapsidation of retroviral RNAs.
-{{STRUCTURE_1f5u|  PDB=1f5u  |  SCENE=  }}
+A retroviral RNA kissing complex containing only two G.C base pairs.,Kim CH, Tinoco I Jr Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9396-401. PMID:10931958<ref>PMID:10931958</ref>
-===SOLUTION STRUCTURE OF THE KISSING DIMER OF H3 GACG STEM-LOOP IN THE 5'-END DIMERIZATION SIGNAL OF MOLONEY MURINE LEUKEMIA VIRUS GENOMIC RNA===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+== References ==
-==About this Structure==
+<references/>
-[[1f5u]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F5U OCA].
+__TOC__
+</StructureSection>
-==Reference==
+[[Category: Kim, C H]]
-<ref group="xtra">PMID:010931958</ref><references group="xtra"/>
+[[Category: Tinoco, I]]
-[[Category: Kim, C H.]]
-[[Category: Tinoco, I.]]
 [[Category: Dimerization]]
 [[Category: Kissing dimer]]