2m4k: Difference between revisions

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{{STRUCTURE_2m4k|  PDB=2m4k  |  SCENE=  }}
==Solution structure of the delta subunit of RNA polymerase from Bacillus subtilis==
===Solution structure of the delta subunit of RNA polymerase from Bacillus subtilis===
<StructureSection load='2m4k' size='340' side='right' caption='[[2m4k]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
{{ABSTRACT_PUBMED_23868186}}
== Structural highlights ==
<table><tr><td colspan='2'>[[2m4k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M4K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2M4K FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rpoE, BSU37160 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 Bacillus subtilis subsp. subtilis str. 168])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2m4k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m4k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2m4k RCSB], [http://www.ebi.ac.uk/pdbsum/2m4k PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The partially disordered delta subunit of RNA polymerase was studied by various NMR techniques. The structure of the well-folded N-terminal domain was determined based on inter-proton distances in NOESY spectra. The obtained structural model was compared to the previously determined structure of a truncated construct (lacking the C-terminal domain). Only marginal differences were identified, thus indicating that the first structural model was not significantly compromised by the absence of the C-terminal domain. Various (15) N relaxation experiments were employed to describe the flexibility of both domains. The relaxation data revealed that the C-terminal domain is more flexible, but its flexibility is not uniform. By using paramagnetic labels, transient contacts of the C-terminal tail with the N-terminal domain and with itself were identified. A propensity of the C-terminal domain to form beta-type structures was obtained by chemical shift analysis. Comparison with the paramagnetic relaxation enhancement indicated a well-balanced interplay of repulsive and attractive electrostatic interactions governing the conformational behavior of the C-terminal domain. The results showed that the delta subunit consists of a well-ordered N-terminal domain and a flexible C-terminal domain that exhibits a complex hierarchy of partial ordering.


==Function==
Structural Study of the Partially Disordered Full-Length delta Subunit of RNA Polymerase from Bacillus subtilis.,Papouskova V, Kaderavek P, Otrusinova O, Rabatinova A, Sanderova H, Novacek J, Krasny L, Sklenar V, Zidek L Chembiochem. 2013 Sep 23;14(14):1772-9. doi: 10.1002/cbic.201300226. Epub 2013, Jul 18. PMID:23868186<ref>PMID:23868186</ref>
[[http://www.uniprot.org/uniprot/RPOE_BACSU RPOE_BACSU]] Participates in both the initiation and recycling phases of transcription. In the presence of the delta subunit, RNAP displays an increased specificity of transcription, a decreased affinity for nucleic acids, and an increased efficiency of RNA synthesis because of enhanced recycling. May function in sigma factor switching. It displaces RNA bound to RNA polymerase in a binary complex.  


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[2m4k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M4K OCA].
</div>


==Reference==
==See Also==
<ref group="xtra">PMID:023868186</ref><references group="xtra"/><references/>
*[[RNA polymerase|RNA polymerase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacillus subtilis subsp. subtilis str. 168]]
[[Category: Bacillus subtilis subsp. subtilis str. 168]]
[[Category: Kaderavek, P.]]
[[Category: Kaderavek, P]]
[[Category: Krasny, L.]]
[[Category: Krasny, L]]
[[Category: Novacek, J.]]
[[Category: Novacek, J]]
[[Category: Papouskova, V.]]
[[Category: Papouskova, V]]
[[Category: Rabatinova, A.]]
[[Category: Rabatinova, A]]
[[Category: Sanderova, H.]]
[[Category: Sanderova, H]]
[[Category: Sklenar, V.]]
[[Category: Sklenar, V]]
[[Category: Zidek, L.]]
[[Category: Zidek, L]]
[[Category: Delta subunit]]
[[Category: Delta subunit]]
[[Category: Rna polymerase]]
[[Category: Rna polymerase]]
[[Category: Transferase]]
[[Category: Transferase]]
Retrieved from "https://proteopedia.org/2m4k"