1tks: Difference between revisions
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[[Image:1tks.gif|left|200px]] | [[Image:1tks.gif|left|200px]] | ||
'''Crystal structure of 3,4-Dihydroxy-2-butanone 4-phosphate Synthase of Candida albicans''' | {{Structure | ||
|PDB= 1tks |SIZE=350|CAPTION= <scene name='initialview01'>1tks</scene>, resolution 1.6Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= CARib3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=237561 Candida albicans SC5314]) | |||
}} | |||
'''Crystal structure of 3,4-Dihydroxy-2-butanone 4-phosphate Synthase of Candida albicans''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1TKS is a [ | 1TKS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Candida_albicans_sc5314 Candida albicans sc5314]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TKS OCA]. | ||
==Reference== | ==Reference== | ||
Potential anti-infective targets in pathogenic yeasts: structure and properties of 3,4-dihydroxy-2-butanone 4-phosphate synthase of Candida albicans., Echt S, Bauer S, Steinbacher S, Huber R, Bacher A, Fischer M, J Mol Biol. 2004 Aug 20;341(4):1085-96. PMID:[http:// | Potential anti-infective targets in pathogenic yeasts: structure and properties of 3,4-dihydroxy-2-butanone 4-phosphate synthase of Candida albicans., Echt S, Bauer S, Steinbacher S, Huber R, Bacher A, Fischer M, J Mol Biol. 2004 Aug 20;341(4):1085-96. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15328619 15328619] | ||
[[Category: Candida albicans sc5314]] | [[Category: Candida albicans sc5314]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: 3]] | [[Category: 3]] | ||
[[Category: 4-dihydroxy-2-butanone 4-phosphate synthase]] | [[Category: 4-dihydroxy-2-butanone 4-phosphate synthase]] | ||
[[Category: candida | [[Category: candida albican]] | ||
[[Category: crystal structure synthetic gene]] | [[Category: crystal structure synthetic gene]] | ||
[[Category: riboflavin biosynthesis]] | [[Category: riboflavin biosynthesis]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:19:42 2008'' | ||
Revision as of 12:19, 20 March 2008
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| 1tks, resolution 1.6Å | |||||||||||||
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| Gene: | CARib3 (Candida albicans SC5314) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of 3,4-Dihydroxy-2-butanone 4-phosphate Synthase of Candida albicans
Overview
A synthetic gene specifying a putative 3,4-dihydroxy-2-butanone 4-phosphate synthase of Candida albicans directed the synthesis of a 22.5 kDa peptide in a recombinant Escherichia coli strain. The recombinant protein was purified to apparent homogeneity by two chromatographic steps and was shown to catalyze the formation of L-3,4-dihydroxy-2-butanone 4-phosphate from ribulose 5-phosphate at a rate of 332 nmol mg(-1) min(-1). Hydrodynamic studies indicated a native molecular mass of 41 kDa in line with a homodimer structure. The protein was crystallized in its apoform. Soaking yielded crystals in complex with the substrate ribulose 5-phosphate. The structures were solved at resolutions of 1.6 and 1.7 angstroms, respectively, using 3,4-dihydroxy-2-butanone 4-phosphate synthase of E. coli for molecular replacement. Structural comparison with the orthologs of Magnaporthe grisea and Methanococcus jannaschii revealed a hitherto unknown conformation of the essential acidic active-site loop.
About this Structure
1TKS is a Single protein structure of sequence from Candida albicans sc5314. Full crystallographic information is available from OCA.
Reference
Potential anti-infective targets in pathogenic yeasts: structure and properties of 3,4-dihydroxy-2-butanone 4-phosphate synthase of Candida albicans., Echt S, Bauer S, Steinbacher S, Huber R, Bacher A, Fischer M, J Mol Biol. 2004 Aug 20;341(4):1085-96. PMID:15328619
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