1u3e: Difference between revisions
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[[Image:1u3e.gif|left|200px]] | [[Image:1u3e.gif|left|200px]] | ||
'''DNA binding and cleavage by the HNH homing endonuclease I-HmuI''' | {{Structure | ||
|PDB= 1u3e |SIZE=350|CAPTION= <scene name='initialview01'>1u3e</scene>, resolution 2.92Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SR:STRONTIUM+ION'>SR</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene> and <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene> | |||
|ACTIVITY= | |||
|GENE= bacteriophage SP01 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=194966 Enterobacteria phage SP6]) | |||
}} | |||
'''DNA binding and cleavage by the HNH homing endonuclease I-HmuI''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1U3E is a [ | 1U3E is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_sp6 Enterobacteria phage sp6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U3E OCA]. | ||
==Reference== | ==Reference== | ||
DNA binding and cleavage by the HNH homing endonuclease I-HmuI., Shen BW, Landthaler M, Shub DA, Stoddard BL, J Mol Biol. 2004 Sep 3;342(1):43-56. PMID:[http:// | DNA binding and cleavage by the HNH homing endonuclease I-HmuI., Shen BW, Landthaler M, Shub DA, Stoddard BL, J Mol Biol. 2004 Sep 3;342(1):43-56. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15313606 15313606] | ||
[[Category: Enterobacteria phage sp6]] | [[Category: Enterobacteria phage sp6]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:26:51 2008'' | ||
Revision as of 12:26, 20 March 2008
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| 1u3e, resolution 2.92Å | |||||||||||||
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| Ligands: | MN, SR, EDO and TRS | ||||||||||||
| Gene: | bacteriophage SP01 (Enterobacteria phage SP6) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
DNA binding and cleavage by the HNH homing endonuclease I-HmuI
Overview
The structure of I-HmuI, which represents the last family of homing endonucleases without a defining crystallographic structure, has been determined in complex with its DNA target. A series of diverse protein structural domains and motifs, contacting sequential stretches of nucleotide bases, are distributed along the DNA target. I-HmuI contains an N-terminal domain with a DNA-binding surface found in the I-PpoI homing endonuclease and an associated HNH/N active site found in the bacterial colicins, and a C-terminal DNA-binding domain previously observed in the I-TevI homing endonuclease. The combination and exchange of these features between protein families indicates that the genetic mobility associated with homing endonucleases extends to the level of independent structural domains. I-HmuI provides an unambiguous structural connection between the His-Cys box endonucleases and the bacterial colicins, supporting the hypothesis that these enzymes diverged from a common ancestral nuclease.
About this Structure
1U3E is a Single protein structure of sequence from Enterobacteria phage sp6. Full crystallographic information is available from OCA.
Reference
DNA binding and cleavage by the HNH homing endonuclease I-HmuI., Shen BW, Landthaler M, Shub DA, Stoddard BL, J Mol Biol. 2004 Sep 3;342(1):43-56. PMID:15313606
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