1uis: Difference between revisions
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'''The 2.0 crystal structure of eqFP611, a far-red fluorescent protein from the sea anemone Entacmaea quadricolor''' | {{Structure | ||
|PDB= 1uis |SIZE=350|CAPTION= <scene name='initialview01'>1uis</scene>, resolution 2.0Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=ACY:ACETIC ACID'>ACY</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''The 2.0 crystal structure of eqFP611, a far-red fluorescent protein from the sea anemone Entacmaea quadricolor''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1UIS is a [ | 1UIS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Entacmaea_quadricolor Entacmaea quadricolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UIS OCA]. | ||
==Reference== | ==Reference== | ||
The 2.0-A crystal structure of eqFP611, a far red fluorescent protein from the sea anemone Entacmaea quadricolor., Petersen J, Wilmann PG, Beddoe T, Oakley AJ, Devenish RJ, Prescott M, Rossjohn J, J Biol Chem. 2003 Nov 7;278(45):44626-31. Epub 2003 Aug 8. PMID:[http:// | The 2.0-A crystal structure of eqFP611, a far red fluorescent protein from the sea anemone Entacmaea quadricolor., Petersen J, Wilmann PG, Beddoe T, Oakley AJ, Devenish RJ, Prescott M, Rossjohn J, J Biol Chem. 2003 Nov 7;278(45):44626-31. Epub 2003 Aug 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12909624 12909624] | ||
[[Category: Entacmaea quadricolor]] | [[Category: Entacmaea quadricolor]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: luminescent protein]] | [[Category: luminescent protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:32:32 2008'' | ||
Revision as of 12:32, 20 March 2008
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| 1uis, resolution 2.0Å | |||||||||||||
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| Ligands: | CA and ACY | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
The 2.0 crystal structure of eqFP611, a far-red fluorescent protein from the sea anemone Entacmaea quadricolor
Overview
We have crystallized and subsequently determined to 2.0-A resolution the crystal structure of eqFP611, a far red fluorescent protein from the sea anemone Entacmaea quadricolor. The structure of the protomer, which adopts a beta-can topology, is similar to that of the related monomeric green fluorescent protein (GFP). The quaternary structure of eqFP611, a tetramer exhibiting 222 symmetry, is similar to that observed for the more closely related red fluorescent protein DsRed and the chromoprotein Rtms5. The unique chromophore sequence (Met63-Tyr64-Gly65) of eqFP611, adopts a coplanar and trans conformation within the interior of the beta-can fold. Accordingly, the eqFP611 chromophore adopts a significantly different conformation in comparison to the chromophore conformation observed in GFP, DsRed, and Rtms5. The coplanar chromophore conformation and its immediate environment provide a structural basis for the far red, highly fluorescent nature of eqFP611. The eqFP611 structure extends our knowledge on the range of conformations a chromophore can adopt within closely related members of the green fluorescent protein family.
About this Structure
1UIS is a Single protein structure of sequence from Entacmaea quadricolor. Full crystallographic information is available from OCA.
Reference
The 2.0-A crystal structure of eqFP611, a far red fluorescent protein from the sea anemone Entacmaea quadricolor., Petersen J, Wilmann PG, Beddoe T, Oakley AJ, Devenish RJ, Prescott M, Rossjohn J, J Biol Chem. 2003 Nov 7;278(45):44626-31. Epub 2003 Aug 8. PMID:12909624
Page seeded by OCA on Thu Mar 20 14:32:32 2008
