1vbk: Difference between revisions
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'''Crystal structure of PH1313 from Pyrococcus horikoshii Ot3''' | {{Structure | ||
|PDB= 1vbk |SIZE=350|CAPTION= <scene name='initialview01'>1vbk</scene>, resolution 1.90Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Crystal structure of PH1313 from Pyrococcus horikoshii Ot3''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1VBK is a [ | 1VBK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VBK OCA]. | ||
==Reference== | ==Reference== | ||
Purification, crystallization and preliminary crystallographic analysis of the putative thiamine-biosynthesis protein PH1313 from Pyrococcus horikoshii OT3., Sugahara M, Murai S, Sugahara M, Kunishima N, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Jan 1;63(Pt, 1):56-8. Epub 2006 Dec 22. PMID:[http:// | Purification, crystallization and preliminary crystallographic analysis of the putative thiamine-biosynthesis protein PH1313 from Pyrococcus horikoshii OT3., Sugahara M, Murai S, Sugahara M, Kunishima N, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Jan 1;63(Pt, 1):56-8. Epub 2006 Dec 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17183176 17183176] | ||
[[Category: Pyrococcus horikoshii]] | [[Category: Pyrococcus horikoshii]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Sugahara, M.]] | [[Category: Sugahara, M.]] | ||
[[Category: MRD]] | [[Category: MRD]] | ||
[[Category: national project on protein structural and functional | [[Category: national project on protein structural and functional analyse]] | ||
[[Category: nppsfa]] | [[Category: nppsfa]] | ||
[[Category: pyrococcus horikoshii]] | [[Category: pyrococcus horikoshii]] | ||
[[Category: riken structural genomics/proteomics initiative]] | [[Category: riken structural genomics/proteomics initiative]] | ||
[[Category: rsgi]] | [[Category: rsgi]] | ||
[[Category: structural | [[Category: structural genomic]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:43:24 2008'' | ||
Revision as of 12:43, 20 March 2008
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| 1vbk, resolution 1.90Å | |||||||||||||
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| Ligands: | MRD | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of PH1313 from Pyrococcus horikoshii Ot3
Overview
The putative thiamine-biosynthesis protein PH1313 from Pyrococcus horikoshii OT3 has been overexpressed and purified. Crystallization was performed by the oil-microbatch method using 28%(v/v) 2-methyl-2,4-pentanediol as a precipitant at 291 K. A native X-ray diffraction data set at 1.9 A resolution and a single anomalous dispersion data set from a selenomethionine-derivative crystal at 2.1 A resolution were collected using synchrotron radiation at 100 K. The native crystal belongs to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 71.7, b = 71.2, c = 141.8 A.
About this Structure
1VBK is a Single protein structure of sequence from Pyrococcus horikoshii. Full crystallographic information is available from OCA.
Reference
Purification, crystallization and preliminary crystallographic analysis of the putative thiamine-biosynthesis protein PH1313 from Pyrococcus horikoshii OT3., Sugahara M, Murai S, Sugahara M, Kunishima N, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Jan 1;63(Pt, 1):56-8. Epub 2006 Dec 22. PMID:17183176
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