Sandbox Reserved 960: Difference between revisions

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The protein AmelASP1 has been identified in the antennae from the honeybee A.mellifera. Its primary sequence is a 144 amino acids polypeptide with a molecular weight of 13.180 kDa.

The protein AmelASP1 has been identified in the antennae from the honeybee A.mellifera. Its primary sequence is a 144 amino acids polypeptide with a molecular weight of 13.180 kDa. AmelASP1 is part of the Pheromone Binding Protein (PBP) family. The 3D representation shown below was obtained at pH 5.5 using the nano-drops technique.

AmelASP1 is part of the Pheromone Binding Protein (PBP) family.

The 3D representation shown below was obtained at pH 5.5 using the nano-drops technique.

=Biological function=

As many other social insects, honeybees employ a large varsity of pheromones to ensure intraspecific communication in several behavioral contexts.

The social organization of the hive is strongly determined by chemical signals, also known as pheromones, that are actively produced and transmitted by the queen.

==Social relevance==

The diet of workers bees and queen bee is strongly different and determinates diverse behaviours.

Workers bee are fed with royal jelly for only three days after egg-laying whereas the queen bee eats royal jelly during her whole life. She controls the activity of each bees by chemical communication.

Actually, the queen bee is the only one able to produce 9-ODA - the main component of its pheromone which induces sexual or endocrine responses. This substance is sent to the workers bees which detect it through pheromone-binding proteins (PBPs). It is then transformed in 9-HDA and added in the royal jelly. This last substance is eaten by the queen. In turn, queen bee transforms 9-HDA into 9-ODA.

Thus, ASP1 is primordial to the internal pheromon’s transport cycle in the hive. By binding the component of queen bee pheromon, bees blend essential behaviour for the swarm. Indeed, 9-ODA is responsible, among others, of preventing workers bees’ ovarian developpment.

In order to determine this proteine’s structure, a serendipitous ligand has been used at pH 5.5. This low pH fit with the natural medium of this protein in the bee antenna.

Sandbox Reserved 960: Difference between revisions

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 <nowiki>
-The protein AmelASP1 has been identified in the antennae from the honeybee A.mellifera. Its primary sequence is a 144 amino acids polypeptide with a molecular weight of 13.180 kDa.
+The protein AmelASP1 has been identified in the antennae from the honeybee A.mellifera. Its primary sequence is a 144 amino acids polypeptide with a molecular weight of 13.180 kDa. AmelASP1 is part of the Pheromone Binding Protein (PBP) family. The 3D representation shown below was obtained at pH 5.5 using the nano-drops technique.
-AmelASP1 is part of the Pheromone Binding Protein (PBP) family.
-The 3D representation shown below was obtained at pH 5.5 using the nano-drops technique.
+=Biological function=
+As many other social insects, honeybees employ a large varsity of pheromones to ensure intraspecific communication in several behavioral contexts.
+The social organization of the hive is strongly determined by chemical signals, also known as pheromones, that are actively produced and transmitted by the queen.
+==Social relevance==
+The diet of workers bees and queen bee is strongly different and determinates diverse behaviours.
+Workers bee are fed with royal jelly for only three days after egg-laying whereas the queen bee eats royal jelly during her whole life. She controls the activity of each bees by chemical communication.
+Actually, the queen bee is the only one able to produce 9-ODA - the main component of its pheromone which induces sexual or endocrine responses. This substance is sent to the workers bees which detect it through pheromone-binding proteins (PBPs). It is then transformed in 9-HDA and added in the royal jelly. This last substance is eaten by the queen. In turn, queen bee transforms 9-HDA into 9-ODA.
+Thus, ASP1 is primordial to the internal pheromon’s transport cycle in the hive. By binding the component of queen bee pheromon, bees blend essential behaviour for the swarm. Indeed, 9-ODA is responsible, among others, of preventing workers bees’ ovarian developpment.
+In order to determine this proteine’s structure, a serendipitous ligand has been used at pH 5.5. This low pH fit with the natural medium of this protein in the bee antenna.
 <ref>PMID: 18508083</ref>