4lzf: Difference between revisions
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==A novel domain in the microcephaly protein CPAP suggests a role in centriole architecture== | |||
<StructureSection load='4lzf' size='340' side='right' caption='[[4lzf]], [[Resolution|resolution]] 1.72Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4lzf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Brachidanio_rerio Brachidanio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LZF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LZF FirstGlance]. <br> | |||
==Function== | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ld1|4ld1]], [[4ld3|4ld3]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cenpj ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7955 Brachidanio rerio])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lzf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lzf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4lzf RCSB], [http://www.ebi.ac.uk/pdbsum/4lzf PDBsum]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/STIL_DANRE STIL_DANRE]] Plays an essential role in early embryonic development. | [[http://www.uniprot.org/uniprot/STIL_DANRE STIL_DANRE]] Plays an essential role in early embryonic development. | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Centrioles are evolutionarily conserved eukaryotic organelles composed of a protein scaffold surrounded by sets of microtubules organized with a 9-fold radial symmetry. CPAP, a centriolar protein essential for microtubule recruitment, features a C-terminal domain of unknown structure, the G-box. A missense mutation in the G-box reduces affinity for the centriolar shuttling protein STIL and causes primary microcephaly. Here, we characterize the molecular architecture of CPAP and determine the G-box structure alone and in complex with a STIL fragment. The G-box comprises a single elongated beta sheet capable of forming supramolecular assemblies. Structural and biophysical studies highlight the conserved nature of the CPAP-STIL complex. We propose that CPAP acts as a horizontal "strut" that joins the centriolar scaffold with microtubules, whereas G-box domains form perpendicular connections. | |||
Structural Analysis of the G-Box Domain of the Microcephaly Protein CPAP Suggests a Role in Centriole Architecture.,Hatzopoulos GN, Erat MC, Cutts E, Rogala KB, Slater LM, Stansfeld PJ, Vakonakis I Structure. 2013 Sep 24. pii: S0969-2126(13)00340-7. doi:, 10.1016/j.str.2013.08.019. PMID:24076405<ref>PMID:24076405</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Brachidanio rerio]] | [[Category: Brachidanio rerio]] | ||
[[Category: Cutts, E | [[Category: Cutts, E]] | ||
[[Category: Erat, M C | [[Category: Erat, M C]] | ||
[[Category: Hatzopoulos, G N | [[Category: Hatzopoulos, G N]] | ||
[[Category: Rogala, K]] | [[Category: Rogala, K]] | ||
[[Category: Slatter, L | [[Category: Slatter, L]] | ||
[[Category: Stansfeld, P J | [[Category: Stansfeld, P J]] | ||
[[Category: Vakonakis, I | [[Category: Vakonakis, I]] | ||
[[Category: Beta-sheet]] | [[Category: Beta-sheet]] | ||
[[Category: Centriole organisation]] | [[Category: Centriole organisation]] | ||
Revision as of 06:45, 24 December 2014
A novel domain in the microcephaly protein CPAP suggests a role in centriole architecture
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