3whj: Difference between revisions
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==Crystal structure of Nas2 N-terminal domain== | |||
=== | <StructureSection load='3whj' size='340' side='right' caption='[[3whj]], [[Resolution|resolution]] 1.65Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3whj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WHJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WHJ FirstGlance]. <br> | |||
==Function== | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3whk|3whk]], [[3whl|3whl]], [[3vld|3vld]], [[3vle|3vle]], [[3vlf|3vlf]], [[3acp|3acp]], [[2dzn|2dzn]], [[3h4m|3h4m]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NAS2, YIL007C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3whj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3whj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3whj RCSB], [http://www.ebi.ac.uk/pdbsum/3whj PDBsum]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/PSMD9_YEAST PSMD9_YEAST]] Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the 19S regulatory complex (RC). During the base subcomplex assembly is part of a NAS2:RPT4:RPT5 module; NAS2 is released during the further base assembly process. | [[http://www.uniprot.org/uniprot/PSMD9_YEAST PSMD9_YEAST]] Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the 19S regulatory complex (RC). During the base subcomplex assembly is part of a NAS2:RPT4:RPT5 module; NAS2 is released during the further base assembly process. | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Proteasome formation does not occur due to spontaneous self-organization but results from a highly ordered process assisted by several assembly chaperones. The assembly of the proteasome ATPase subunits is assisted by four client-specific chaperones, of which three have been structurally resolved. Here, we provide the structural basis for the working mechanisms of the last, hereto structurally uncharacterized assembly chaperone, Nas2. We revealed that Nas2 binds to the Rpt5 subunit in a bivalent mode: the N-terminal helical domain of Nas2 masks the Rpt1-interacting surface of Rpt5, whereas its C-terminal PDZ domain caps the C-terminal proteasome-activating motif. Thus, Nas2 operates as a proteasome activation blocker, offering a checkpoint during the formation of the 19S ATPase prior to its docking onto the proteolytic 20S core particle. | |||
Structural Basis for Proteasome Formation Controlled by an Assembly Chaperone Nas2.,Satoh T, Saeki Y, Hiromoto T, Wang YH, Uekusa Y, Yagi H, Yoshihara H, Yagi-Utsumi M, Mizushima T, Tanaka K, Kato K Structure. 2014 Mar 25. pii: S0969-2126(14)00070-7. doi:, 10.1016/j.str.2014.02.014. PMID:24685148<ref>PMID:24685148</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Baker's yeast]] | [[Category: Baker's yeast]] | ||
[[Category: Hiromoto, T | [[Category: Hiromoto, T]] | ||
[[Category: Kato, K | [[Category: Kato, K]] | ||
[[Category: Mizushima, T | [[Category: Mizushima, T]] | ||
[[Category: Saeki, Y | [[Category: Saeki, Y]] | ||
[[Category: Satoh, T | [[Category: Satoh, T]] | ||
[[Category: Tanaka, K | [[Category: Tanaka, K]] | ||
[[Category: Uekusa, Y | [[Category: Uekusa, Y]] | ||
[[Category: Wang, Y H | [[Category: Wang, Y H]] | ||
[[Category: Yagi, H | [[Category: Yagi, H]] | ||
[[Category: Yagi-Utsumi, M | [[Category: Yagi-Utsumi, M]] | ||
[[Category: Yoshihara, H | [[Category: Yoshihara, H]] | ||
[[Category: Chaperone]] | [[Category: Chaperone]] | ||
[[Category: Proteasome assembly chaperone]] | [[Category: Proteasome assembly chaperone]] | ||
