Sandbox Reserved 960: Difference between revisions

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The protein AmelASP1 has been identified in the antennae from the honeybee ''Apis mellifera''. Its primary sequence is a 144 amino acids polypeptide with a molecular weight of 13.180 kDa. AmelASP1 is part of the Pheromone Binding Protein (PBP) family. The 3D representation shown below was obtained at pH 5.5 using the ~~[http://www-dsv.cea.fr/en/life-science-div/all-the-news/scientific-results/nanodrops-for-bioactive-compound-synthesis-and-screening~~ nano-drops technique].

The protein AmelASP1 has been identified in the antennae from the honeybee ''Apis mellifera''. Its primary sequence is a 144 amino acids polypeptide with a molecular weight of 13.180 kDa. AmelASP1 is part of the Pheromone Binding Protein (PBP) family. The 3D representation shown below was obtained at pH 5.5 using the nano-drops technique.

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The diets of workers bees and queen bee are strongly different and determinate diverse behaviors.

Workers bees are fed with royal jelly for only three days after egg-laying whereas the queen bee eats royal jelly during her whole life. She controls the activity of each bees by chemical communication.

Actually, the queen bee is the only one able to produce ~~[http://www.chemspider.com/Chemical-Structure.1362276.html~~ 9-ODA] - the main component of its pheromone which induces sexual or endocrine responses. This substance is sent to the workers bees which detect it through pheromone-binding proteins (PBPs). It is then transformed in ~~[http://www.chemspider.com/Chemical-Structure.4472227.html~~ 9-HDA] and added in the royal jelly. This last substance is eaten by the queen. In turn, queen bee transforms 9-HDA into 9-ODA.

Actually, the queen bee is the only one able to produce 9-ODA - the main component of its pheromone which induces sexual or endocrine responses. This substance is sent to the workers bees which detect it through pheromone-binding proteins (PBPs). It is then transformed in 9-HDA and added in the royal jelly. This last substance is eaten by the queen. In turn, queen bee transforms 9-HDA into 9-ODA.

Thus, ASP1 is primordial to the internal pheromone’s transport cycle in the hive. By binding the component of queen bee pheromone, bees express and transmit essential behaviour within the hive. Indeed, 9-ODA is responsible, among others, of preventing workers bees’ ovarian development.

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=== Components implicated in the structure rigidity ===

AmelASP1 presents <scene name='60/604479/Disulfide_bonds/1'> three disulfide bridges</scene> which are greatly enhancing its structure’s rigidity by linking four of the helices together. The six ~~cysteines~~ and their interval spacing are the most striking features shared by proteins belonging to the PBP family.

AmelASP1 presents <scene name='60/604479/Disulfide_bonds/1'> three disulfide bridges</scene> which are greatly enhancing its structure’s rigidity by linking four of the helices together. The <scene name='60/604479/Six_conserved_cysteins/1'>six cysteins</scene> and their interval spacing are the most striking features shared by proteins belonging to the PBP family.

The <scene name='60/604479/1st_disulfide_bridge/1'>first disulfide bridge</scene> is established between <scene name='60/604479/H1/2'>H1</scene> and <scene name='60/604479/H3/2'>H3</scene> through Cysteines 20 and 51. <scene name='60/604479/2nd_disulfide_bridge/2'>An other disulfide bridge</scene> links <scene name='60/604479/H3/2'>H3</scene> and <scene name='60/604479/H6/1'>H6</scene> through Cys 47 and 98, and the <scene name='60/604479/3rd_disulfide_bridge/1'>third disulfide bridge</scene> connects <scene name='60/604479/H5/1'>H5</scene> and <scene name='60/604479/H6/1'>H6</scene> thanks to Cys 89 and Cys 107.

Sandbox Reserved 960: Difference between revisions

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@@ Line 5: / Line 5: @@
 <nowiki>
-The protein AmelASP1 has been identified in the antennae from the honeybee ''Apis mellifera''. Its primary sequence is a 144 amino acids polypeptide with a molecular weight of 13.180 kDa. AmelASP1 is part of the Pheromone Binding Protein (PBP) family. The 3D representation shown below was obtained at pH 5.5 using the [http://www-dsv.cea.fr/en/life-science-div/all-the-news/scientific-results/nanodrops-for-bioactive-compound-synthesis-and-screening nano-drops technique].
+The protein AmelASP1 has been identified in the antennae from the honeybee ''Apis mellifera''. Its primary sequence is a 144 amino acids polypeptide with a molecular weight of 13.180 kDa. AmelASP1 is part of the Pheromone Binding Protein (PBP) family. The 3D representation shown below was obtained at pH 5.5 using the nano-drops technique.
@@ Line 15: / Line 15: @@
 The diets of workers bees and queen bee are strongly different and determinate diverse behaviors.
 Workers bees are fed with royal jelly for only three days after egg-laying whereas the queen bee eats royal jelly during her whole life. She controls the activity of each bees by chemical communication.
-Actually, the queen bee is the only one able to produce [http://www.chemspider.com/Chemical-Structure.1362276.html 9-ODA] - the main component of its pheromone which induces sexual or endocrine responses. This substance is sent to the workers bees which detect it through pheromone-binding proteins (PBPs). It is then transformed in [http://www.chemspider.com/Chemical-Structure.4472227.html 9-HDA] and added in the royal jelly. This last substance is eaten by the queen. In turn, queen bee transforms 9-HDA into 9-ODA.
+Actually, the queen bee is the only one able to produce 9-ODA - the main component of its pheromone which induces sexual or endocrine responses. This substance is sent to the workers bees which detect it through pheromone-binding proteins (PBPs). It is then transformed in 9-HDA and added in the royal jelly. This last substance is eaten by the queen. In turn, queen bee transforms 9-HDA into 9-ODA.
 Thus, ASP1 is primordial to the internal pheromone’s transport cycle in the hive. By binding the component of queen bee pheromone, bees express and transmit essential behaviour within the hive. Indeed, 9-ODA is responsible, among others, of preventing workers bees’ ovarian development.
@@ Line 39: / Line 39: @@
 === Components implicated in the structure rigidity ===
-AmelASP1 presents <scene name='60/604479/Disulfide_bonds/1'> three disulfide bridges</scene> which are greatly enhancing its structure’s rigidity by linking four of the helices together. The six cysteines and their interval spacing are the most striking features shared by proteins belonging to the PBP family.
+AmelASP1 presents <scene name='60/604479/Disulfide_bonds/1'> three disulfide bridges</scene> which are greatly enhancing its structure’s rigidity by linking four of the helices together. The <scene name='60/604479/Six_conserved_cysteins/1'>six cysteins</scene> and their interval spacing are the most striking features shared by proteins belonging to the PBP family.
 The <scene name='60/604479/1st_disulfide_bridge/1'>first disulfide bridge</scene> is established between <scene name='60/604479/H1/2'>H1</scene> and <scene name='60/604479/H3/2'>H3</scene> through Cysteines 20 and 51. <scene name='60/604479/2nd_disulfide_bridge/2'>An other disulfide bridge</scene>  links <scene name='60/604479/H3/2'>H3</scene> and <scene name='60/604479/H6/1'>H6</scene> through Cys 47 and 98, and the <scene name='60/604479/3rd_disulfide_bridge/1'>third disulfide bridge</scene> connects <scene name='60/604479/H5/1'>H5</scene> and <scene name='60/604479/H6/1'>H6</scene> thanks to Cys 89 and Cys 107.