1obc: Difference between revisions
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==Overview== | ==Overview== | ||
The aminoacyl-tRNA synthetases link tRNAs with their cognate amino acid., In some cases, their fidelity relies on hydrolytic editing that destroys, incorrectly activated amino acids or mischarged tRNAs. We present, structures of leucyl-tRNA synthetase complexed with analogs of the, distinct pre- and posttransfer editing substrates. The editing active site, binds the two different substrates using a single amino acid, discriminatory pocket while preserving the same mode of adenine, recognition. This suggests a similar mechanism of hydrolysis for both, editing substrates that depends on a key, completely conserved aspartic, acid, which interacts with the alpha-amino group of the noncognate amino, acid and positions both substrates for hydrolysis. Our results demonstrate, the economy by . | The aminoacyl-tRNA synthetases link tRNAs with their cognate amino acid., In some cases, their fidelity relies on hydrolytic editing that destroys, incorrectly activated amino acids or mischarged tRNAs. We present, structures of leucyl-tRNA synthetase complexed with analogs of the, distinct pre- and posttransfer editing substrates. The editing active site, binds the two different substrates using a single amino acid, discriminatory pocket while preserving the same mode of adenine, recognition. This suggests a similar mechanism of hydrolysis for both, editing substrates that depends on a key, completely conserved aspartic, acid, which interacts with the alpha-amino group of the noncognate amino, acid and positions both substrates for hydrolysis. Our results demonstrate, the economy by which a single active site accommodates two distinct, substrates in a proofreading process critical to the fidelity of protein, synthesis. | ||
==About this Structure== | ==About this Structure== | ||
1OBC is a | 1OBC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with ZN, LEU and LMS as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OBC OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: synthetase]] | [[Category: synthetase]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 13:04:10 2007'' | ||
