4eq5: Difference between revisions
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[[ | ==DNA ligase from the archaeon Thermococcus sibiricus== | ||
<StructureSection load='4eq5' size='340' side='right' caption='[[4eq5]], [[Resolution|resolution]] 2.85Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4eq5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermococcus_sibiricus Thermococcus sibiricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EQ5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EQ5 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2cfm|2cfm]], [[3gde|3gde]], [[2hiv|2hiv]], [[3rr5|3rr5]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lig, Thermostable ATP-dependent DNA ligase gene Tsib_0885, TSIB_0885 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=172049 Thermococcus sibiricus])</td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_ligase_(ATP) DNA ligase (ATP)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.5.1.1 6.5.1.1] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eq5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eq5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eq5 RCSB], [http://www.ebi.ac.uk/pdbsum/4eq5 PDBsum]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/C6A2U9_THESM C6A2U9_THESM]] DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.[HAMAP-Rule:MF_00407] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
DNA ligases join single-strand breaks in double-stranded DNA by catalyzing the formation of a phosphodiester bond between adjacent 5'-phosphate and 3'-hydroxyl termini. Their function is essential to maintain the integrity of the genome in DNA replication, recombination and repair. A recombinant ATP-dependent DNA ligase from the hyperthermophilic anaerobic archaeon Thermococcus sibiricus was expressed in Escherichia coli and purified. Crystals were grown by vapour diffusion using the hanging-drop method with 17%(w/v) PEG 4000 and 8.5%(v/v) 2-propanol as precipitants. A diffraction experiment was performed with a single crystal, which diffracted X-rays to 3.0 A resolution. The crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 58.590, b = 87.540, c = 126.300 A. | |||
Expression, purification, crystallization and preliminary crystallographic analysis of a thermostable DNA ligase from the archaeon Thermococcus sibiricus.,Petrova TE, Bezsudnova EY, Dorokhov BD, Slutskaya ES, Polyakov KM, Dorovatovskiy PV, Ravin NV, Skryabin KG, Kovalchuk MV, Popov VO Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Feb 1;68(Pt 2):163-5. Epub, 2012 Jan 25. PMID:22297989<ref>PMID:22297989</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | ==See Also== | ||
*[[DNA ligase|DNA ligase]] | *[[DNA ligase|DNA ligase]] | ||
== References == | |||
== | <references/> | ||
< | __TOC__ | ||
</StructureSection> | |||
[[Category: Thermococcus sibiricus]] | [[Category: Thermococcus sibiricus]] | ||
[[Category: Bezsudnova, E Y | [[Category: Bezsudnova, E Y]] | ||
[[Category: Dorokhov, B D | [[Category: Dorokhov, B D]] | ||
[[Category: Dorovatovskiy, P V | [[Category: Dorovatovskiy, P V]] | ||
[[Category: Kovalchuk, M V | [[Category: Kovalchuk, M V]] | ||
[[Category: Petrova, T | [[Category: Petrova, T]] | ||
[[Category: Polyakov, K M | [[Category: Polyakov, K M]] | ||
[[Category: Popov, V O | [[Category: Popov, V O]] | ||
[[Category: Ravin, N V | [[Category: Ravin, N V]] | ||
[[Category: Skryabin, K G | [[Category: Skryabin, K G]] | ||
[[Category: Slutskaya, E S | [[Category: Slutskaya, E S]] | ||
[[Category: Adenylation domain]] | [[Category: Adenylation domain]] | ||
[[Category: Archaeon thermococcus sibiricus]] | [[Category: Archaeon thermococcus sibiricus]] | ||
Revision as of 16:46, 24 December 2014
DNA ligase from the archaeon Thermococcus sibiricus
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