1yfe: Difference between revisions
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'''Crystal structure of apo fumarase C from Escherichia coli''' | {{Structure | ||
|PDB= 1yfe |SIZE=350|CAPTION= <scene name='initialview01'>1yfe</scene>, resolution 2.19Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Fumarate_hydratase Fumarate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.2 4.2.1.2] | |||
|GENE= | |||
}} | |||
'''Crystal structure of apo fumarase C from Escherichia coli''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1YFE is a [ | 1YFE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YFE OCA]. | ||
==Reference== | ==Reference== | ||
Structure of free fumarase C from Escherichia coli., Weaver T, Acta Crystallogr D Biol Crystallogr. 2005 Oct;61(Pt 10):1395-401. Epub, 2005 Sep 28. PMID:[http:// | Structure of free fumarase C from Escherichia coli., Weaver T, Acta Crystallogr D Biol Crystallogr. 2005 Oct;61(Pt 10):1395-401. Epub, 2005 Sep 28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16204892 16204892] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Fumarate hydratase]] | [[Category: Fumarate hydratase]] | ||
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[[Category: kreb's cycle]] | [[Category: kreb's cycle]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:22:09 2008'' | ||
Revision as of 13:22, 20 March 2008
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| 1yfe, resolution 2.19Å | |||||||||||||
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| Activity: | Fumarate hydratase, with EC number 4.2.1.2 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of apo fumarase C from Escherichia coli
Overview
Previous crystal structures of fumarase C from Escherichia coli have noted two occupied dicarboxylate-binding sites termed the active site and the B site. Here, the first known fumarase C structure is reported in which both sites are unoccupied by bound ligand. This so-called ;free' crystal form shows conservation of the active-site water in a similar orientation to that reported in other fumarase C crystal structures. More importantly, a shift of His129 has been observed at the B site. This new crystallographic information suggests the use of water as a permanent member of the active site and the use of an imidazole-imidazolium conversion to control access at the allosteric B site.
About this Structure
1YFE is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structure of free fumarase C from Escherichia coli., Weaver T, Acta Crystallogr D Biol Crystallogr. 2005 Oct;61(Pt 10):1395-401. Epub, 2005 Sep 28. PMID:16204892
Page seeded by OCA on Thu Mar 20 15:22:09 2008
