1yod: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1yod.gif|left|200px]] | [[Image:1yod.gif|left|200px]] | ||
'''Crystal structure of a water soluble analog of phospholamban''' | {{Structure | ||
|PDB= 1yod |SIZE=350|CAPTION= <scene name='initialview01'>1yod</scene>, resolution 1.80Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Crystal structure of a water soluble analog of phospholamban''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
1YOD is a [ | 1YOD is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YOD OCA]. | ||
==Reference== | ==Reference== | ||
X-ray structure of a water-soluble analog of the membrane protein phospholamban: sequence determinants defining the topology of tetrameric and pentameric coiled coils., Slovic AM, Stayrook SE, North B, Degrado WF, J Mol Biol. 2005 May 6;348(3):777-87. PMID:[http:// | X-ray structure of a water-soluble analog of the membrane protein phospholamban: sequence determinants defining the topology of tetrameric and pentameric coiled coils., Slovic AM, Stayrook SE, North B, Degrado WF, J Mol Biol. 2005 May 6;348(3):777-87. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15826670 15826670] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: DeGrado, W F.]] | [[Category: DeGrado, W F.]] | ||
| Line 19: | Line 28: | ||
[[Category: water-soluble]] | [[Category: water-soluble]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:25:31 2008'' | ||
Revision as of 13:25, 20 March 2008
| |||||||||||||
| 1yod, resolution 1.80Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of a water soluble analog of phospholamban
Overview
Phospholamban (PLB) is a pentameric transmembrane protein that regulates the Ca(2+)-dependent ATPase SERCA2a in sarcoplasmic reticulum membranes. We previously described the computational design of a water-soluble variant of phospholamban, WSPLB, which reproduced many of the structural and functional properties of the native membrane-soluble protein. While the full-length WSPLB forms a pentamer in solution, a truncated variant forms very stable tetramers. To obtain insight into the tetramer-pentamer cytoplasmic switch, we solved the crystal structure of the truncated construct, WSPLB 21-52. This peptide has a heptad sequence repeat with Leu residues at a- and Ile at d-positions from residues 31-52. The crystal structure revealed that WSPLB 21-52 adopted an antiparallel tetrameric coiled coil. This topology contrasts with the parallel topology of an analogue of the coiled-coil of GCN4 with the same Leu(a) Ile(d) repeat. Analysis of these structures revealed how the nature of the partially exposed residues at e- and g-positions influence the topology formed by the bundle. We also constructed a model for the pentameric form of PLB using the coiled-coil parameters derived from a single monomer in the tetrameric structure. This model suggests that both buried and interfacial hydrogen bonds are important for stabilizing the parallel pentamer.
About this Structure
1YOD is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
X-ray structure of a water-soluble analog of the membrane protein phospholamban: sequence determinants defining the topology of tetrameric and pentameric coiled coils., Slovic AM, Stayrook SE, North B, Degrado WF, J Mol Biol. 2005 May 6;348(3):777-87. PMID:15826670
Page seeded by OCA on Thu Mar 20 15:25:31 2008
