1zz7: Difference between revisions
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[[Image:1zz7.gif|left|200px]] | [[Image:1zz7.gif|left|200px]] | ||
'''Crystal Structure of FeII HppE in Complex with Substrate form 1''' | {{Structure | ||
|PDB= 1zz7 |SIZE=350|CAPTION= <scene name='initialview01'>1zz7</scene>, resolution 2.10Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene> and <scene name='pdbligand=S0H:(S)-2-HYDROXYPROPYLPHOSPHONIC ACID'>S0H</scene> | |||
|ACTIVITY= | |||
|GENE= fom4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=43759 Streptomyces wedmorensis]) | |||
}} | |||
'''Crystal Structure of FeII HppE in Complex with Substrate form 1''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1ZZ7 is a [ | 1ZZ7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_wedmorensis Streptomyces wedmorensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZZ7 OCA]. | ||
==Reference== | ==Reference== | ||
Structural insight into antibiotic fosfomycin biosynthesis by a mononuclear iron enzyme., Higgins LJ, Yan F, Liu P, Liu HW, Drennan CL, Nature. 2005 Oct 6;437(7060):838-44. Epub 2005 Jul 13. PMID:[http:// | Structural insight into antibiotic fosfomycin biosynthesis by a mononuclear iron enzyme., Higgins LJ, Yan F, Liu P, Liu HW, Drennan CL, Nature. 2005 Oct 6;437(7060):838-44. Epub 2005 Jul 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16015285 16015285] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Streptomyces wedmorensis]] | [[Category: Streptomyces wedmorensis]] | ||
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[[Category: substrate-enzyme complex]] | [[Category: substrate-enzyme complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:41:35 2008'' | ||
Revision as of 13:41, 20 March 2008
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| 1zz7, resolution 2.10Å | |||||||||||||
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| Ligands: | FE2 and S0H | ||||||||||||
| Gene: | fom4 (Streptomyces wedmorensis) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal Structure of FeII HppE in Complex with Substrate form 1
Overview
The biosynthetic pathway of the clinically important antibiotic fosfomycin uses enzymes that catalyse reactions without precedent in biology. Among these is hydroxypropylphosphonic acid epoxidase, which represents a new subfamily of non-haem mononuclear iron enzymes. Here we present six X-ray structures of this enzyme: the apoenzyme at 2.0 A resolution; a native Fe(II)-bound form at 2.4 A resolution; a tris(hydroxymethyl)aminomethane-Co(II)-enzyme complex structure at 1.8 A resolution; a substrate-Co(II)-enzyme complex structure at 2.5 A resolution; and two substrate-Fe(II)-enzyme complexes at 2.1 and 2.3 A resolution. These structural data lead us to suggest how this enzyme is able to recognize and respond to its substrate with a conformational change that protects the radical-based intermediates formed during catalysis. Comparisons with other family members suggest why substrate binding is able to prime iron for dioxygen binding in the absence of alpha-ketoglutarate (a co-substrate required by many mononuclear iron enzymes), and how the unique epoxidation reaction of hydroxypropylphosphonic acid epoxidase may occur.
About this Structure
1ZZ7 is a Single protein structure of sequence from Streptomyces wedmorensis. Full crystallographic information is available from OCA.
Reference
Structural insight into antibiotic fosfomycin biosynthesis by a mononuclear iron enzyme., Higgins LJ, Yan F, Liu P, Liu HW, Drennan CL, Nature. 2005 Oct 6;437(7060):838-44. Epub 2005 Jul 13. PMID:16015285
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