283d: Difference between revisions
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[[Image:283d.jpg|left|200px]] | [[Image:283d.jpg|left|200px]] | ||
'''A CURVED RNA HELIX INCORPORATING AN INTERNAL LOOP WITH G-A AND A-A NON-WATSON-CRICK BASE PAIRING''' | {{Structure | ||
|PDB= 283d |SIZE=350|CAPTION= <scene name='initialview01'>283d</scene>, resolution 2.300Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=MN:MANGANESE (II) ION'>MN</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''A CURVED RNA HELIX INCORPORATING AN INTERNAL LOOP WITH G-A AND A-A NON-WATSON-CRICK BASE PAIRING''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
283D is a [ | 283D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=283D OCA]. | ||
==Reference== | ==Reference== | ||
A curved RNA helix incorporating an internal loop with G.A and A.A non-Watson-Crick base pairing., Baeyens KJ, De Bondt HL, Pardi A, Holbrook SR, Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):12851-5. PMID:[http:// | A curved RNA helix incorporating an internal loop with G.A and A.A non-Watson-Crick base pairing., Baeyens KJ, De Bondt HL, Pardi A, Holbrook SR, Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):12851-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8917508 8917508] | ||
[[Category: ]] | [[Category: ]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: unusual rna]] | [[Category: unusual rna]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:43:36 2008'' | ||
Revision as of 13:43, 20 March 2008
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| 283d, resolution 2.300Å | |||||||||||||
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| Ligands: | MN | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
A CURVED RNA HELIX INCORPORATING AN INTERNAL LOOP WITH G-A AND A-A NON-WATSON-CRICK BASE PAIRING
Overview
The crystal structure of the RNA dodecamer 5'-GGCC(GAAA)GGCC-3' has been determined from x-ray diffraction data to 2.3-A resolution. In the crystal, these oligomers form double helices around twofold symmetry axes. Four consecutive non-Watson-Crick base pairs make up an internal loop in the middle of the duplex, including sheared G.A pairs and novel asymmetric A.A pairs. This internal loop sequence produces a significant curvature and narrowing of the double helix. The helix is curved by 34 degrees from end to end and the diameter is narrowed by 24% in the internal loop. A Mn2+ ion is bound directly to the N7 of the first guanine in the Watson-Crick region following the internal loop and the phosphate of the preceding residue. This Mn2+ location corresponds to a metal binding site observed in the hammerhead catalytic RNA.
About this Structure
283D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
A curved RNA helix incorporating an internal loop with G.A and A.A non-Watson-Crick base pairing., Baeyens KJ, De Bondt HL, Pardi A, Holbrook SR, Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):12851-5. PMID:8917508 [[Category: ]]
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