2alz: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2alz.gif|left|200px]] | [[Image:2alz.gif|left|200px]] | ||
'''Ternary Complex of hPoli with DNA and dCTP''' | {{Structure | ||
|PDB= 2alz |SIZE=350|CAPTION= <scene name='initialview01'>2alz</scene>, resolution 2.50Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=DCP:2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE'>DCP</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] | |||
|GENE= POLI, RAD30B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |||
}} | |||
'''Ternary Complex of hPoli with DNA and dCTP''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
2ALZ is a [ | 2ALZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ALZ OCA]. | ||
==Reference== | ==Reference== | ||
Human DNA polymerase iota incorporates dCTP opposite template G via a G.C + Hoogsteen base pair., Nair DT, Johnson RE, Prakash L, Prakash S, Aggarwal AK, Structure. 2005 Oct;13(10):1569-77. PMID:[http:// | Human DNA polymerase iota incorporates dCTP opposite template G via a G.C + Hoogsteen base pair., Nair DT, Johnson RE, Prakash L, Prakash S, Aggarwal AK, Structure. 2005 Oct;13(10):1569-77. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16216587 16216587] | ||
[[Category: DNA-directed DNA polymerase]] | [[Category: DNA-directed DNA polymerase]] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
| Line 27: | Line 36: | ||
[[Category: ternary complex]] | [[Category: ternary complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:51:14 2008'' | ||
Revision as of 13:51, 20 March 2008
| |||||||||||||
| 2alz, resolution 2.50Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ligands: | MG and DCP | ||||||||||||
| Gene: | POLI, RAD30B (Homo sapiens) | ||||||||||||
| Activity: | DNA-directed DNA polymerase, with EC number 2.7.7.7 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Ternary Complex of hPoli with DNA and dCTP
Overview
Human DNA polymerase iota (hPoliota), a member of the Y family of DNA polymerases, differs in remarkable ways from other DNA polymerases, incorporating correct nucleotides opposite template purines with a much higher efficiency and fidelity than opposite template pyrimidines. We present here the crystal structure of hPoliota bound to template G and incoming dCTP, which reveals a G.C + Hoogsteen base pair in a DNA polymerase active site. We show that the hPoliota active site has evolved to favor Hoogsteen base pairing, wherein the template sugar is fixed in a cavity that reduces the C1'-C1' distance across the nascent base pair from approximately 10.5 A in other DNA polymerases to 8.6 A in hPoliota. The rotation of G from anti to syn is then largely in response to this curtailed C1'-C1' distance. A G.C+ Hoogsteen base pair suggests a specific mechanism for hPoliota's ability to bypass N(2)-adducted guanines that obstruct replication.
About this Structure
2ALZ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Human DNA polymerase iota incorporates dCTP opposite template G via a G.C + Hoogsteen base pair., Nair DT, Johnson RE, Prakash L, Prakash S, Aggarwal AK, Structure. 2005 Oct;13(10):1569-77. PMID:16216587
Page seeded by OCA on Thu Mar 20 15:51:14 2008
