2avu: Difference between revisions
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[[Image:2avu.gif|left|200px]] | [[Image:2avu.gif|left|200px]] | ||
'''Structure of the Escherichia coli FlhDC complex, a prokaryotic heteromeric regulator of transcription''' | {{Structure | ||
|PDB= 2avu |SIZE=350|CAPTION= <scene name='initialview01'>2avu</scene>, resolution 3.00Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | |||
|ACTIVITY= | |||
|GENE= flhD, flbB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), flhC, flaI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |||
}} | |||
'''Structure of the Escherichia coli FlhDC complex, a prokaryotic heteromeric regulator of transcription''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
2AVU is a [ | 2AVU is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AVU OCA]. | ||
==Reference== | ==Reference== | ||
Structure of the Escherichia coli FlhDC complex, a prokaryotic heteromeric regulator of transcription., Wang S, Fleming RT, Westbrook EM, Matsumura P, McKay DB, J Mol Biol. 2006 Jan 27;355(4):798-808. Epub 2005 Nov 22. PMID:[http:// | Structure of the Escherichia coli FlhDC complex, a prokaryotic heteromeric regulator of transcription., Wang S, Fleming RT, Westbrook EM, Matsumura P, McKay DB, J Mol Biol. 2006 Jan 27;355(4):798-808. Epub 2005 Nov 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16337229 16337229] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: c4-type zinc finger]] | [[Category: c4-type zinc finger]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:54:24 2008'' | ||
Revision as of 13:54, 20 March 2008
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| 2avu, resolution 3.00Å | |||||||||||||
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| Ligands: | ZN | ||||||||||||
| Gene: | flhD, flbB (Escherichia coli), flhC, flaI (Escherichia coli) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Structure of the Escherichia coli FlhDC complex, a prokaryotic heteromeric regulator of transcription
Overview
The hetero-oligomeric complex of the FlhD and FlhC proteins (FlhDC) regulates transcription from several flagellar and non-flagellar operons in bacteria. The crystallographic structure of the Escherichia coli FlhDC complex has been solved to 3.0 A resolution, revealing a hexameric FlhD4FlhC2 assembly. In the complex, each FlhC protomer binds an FlhD2 dimer; the conformation of the dimer in the complex differs significantly from its conformation in the absence of FlhC. FlhC has a novel tertiary fold that includes a heretofore unrecognized zinc-binding site in which the ion is ligated by four cysteine residues. Gel shift experiments show that binding of the FlhDC complex to a cognate promoter bends the DNA by approximately 111 degrees . The structure of the FlhDC complex is compatible with models in which a fragment of operator DNA, at least 48 base-pairs in length, wraps around the complex and bends significantly when binding.
About this Structure
2AVU is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structure of the Escherichia coli FlhDC complex, a prokaryotic heteromeric regulator of transcription., Wang S, Fleming RT, Westbrook EM, Matsumura P, McKay DB, J Mol Biol. 2006 Jan 27;355(4):798-808. Epub 2005 Nov 22. PMID:16337229
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