4jhr: Difference between revisions
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==An auto-inhibited conformation of LGN reveals a distinct interaction mode between GoLoco motifs and TPR motifs== | |||
<StructureSection load='4jhr' size='340' side='right' caption='[[4jhr]], [[Resolution|resolution]] 2.80Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4jhr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JHR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JHR FirstGlance]. <br> | |||
==Function== | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Gpsm2, Lgn, Pins ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jhr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jhr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4jhr RCSB], [http://www.ebi.ac.uk/pdbsum/4jhr PDBsum]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/GPSM2_MOUSE GPSM2_MOUSE]] Plays an important role in spindle pole orientation (By similarity). Interacts and contributes to the functional activity of G(i) alpha proteins. Acts to stabilize the apical complex during neuroblast divisions. | [[http://www.uniprot.org/uniprot/GPSM2_MOUSE GPSM2_MOUSE]] Plays an important role in spindle pole orientation (By similarity). Interacts and contributes to the functional activity of G(i) alpha proteins. Acts to stabilize the apical complex during neuroblast divisions. | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
LGN plays essential roles in asymmetric cell divisions via its N-terminal TPR-motif-mediated binding to mInsc and NuMA. This scaffolding activity requires the release of the autoinhibited conformation of LGN by binding of Galphai to its C-terminal GoLoco (GL) motifs. The interaction between the GL and TPR motifs of LGN represents a distinct GL/target binding mode with an unknown mechanism. Here, we show that two consecutive GL motifs of LGN form a minimal TPR-motif-binding unit. GL12 and GL34 bind to TPR0-3 and TPR4-7, respectively. The crystal structure of a truncated LGN reveals that GL34 forms a pair of parallel alpha helices and binds to the concave surface of TPR4-7, thereby preventing LGN from binding to other targets. Importantly, the GLs bind to TPR motifs with a mode distinct from that observed in the GL/Galphai.GDP complexes. Our results also indicate that multiple and orphan GL motif proteins likely respond to G proteins with distinct mechanisms. | |||
An Autoinhibited Conformation of LGN Reveals a Distinct Interaction Mode between GoLoco Motifs and TPR Motifs.,Pan Z, Zhu J, Shang Y, Wei Z, Jia M, Xia C, Wen W, Wang W, Zhang M Structure. 2013 Jun 4;21(6):1007-17. doi: 10.1016/j.str.2013.04.005. Epub 2013, May 9. PMID:23665171<ref>PMID:23665171</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Jia, M | [[Category: Jia, M]] | ||
[[Category: Pan, Z | [[Category: Pan, Z]] | ||
[[Category: Shang, Y | [[Category: Shang, Y]] | ||
[[Category: Wang, W | [[Category: Wang, W]] | ||
[[Category: Wei, Z | [[Category: Wei, Z]] | ||
[[Category: Wen, W | [[Category: Wen, W]] | ||
[[Category: Xia, C | [[Category: Xia, C]] | ||
[[Category: Zhang, M | [[Category: Zhang, M]] | ||
[[Category: Zhu, J | [[Category: Zhu, J]] | ||
[[Category: Asymmetric cell division]] | [[Category: Asymmetric cell division]] | ||
[[Category: Goloco]] | [[Category: Goloco]] | ||
