2hdz

Crystal Structure Analysis of the UBF HMG box5

spinqualitylabelsall models 2hdz, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2hdz is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: UBF (Homo sapiens) Resources: FirstGlance, OCA, RCSB, PDBsum Function [UBF1_HUMAN] Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element. Publication Abstract from PubMed The fifth HMG-box domain in human upstream binding factor (hUBF) contributes to the synthesis of rRNA by RNA polymerase I (Pol I). The 2.0 A resolution crystal structure of this protein has been solved using the single-wavelength anomalous dispersion method (SAD). The crystal structure and the reported NMR structure have r.m.s. deviations of 2.18-3.03 A for the C(alpha) atoms. However, there are significant differences between the two structures, with displacements of up to 9.0 A. Compared with other HMG-box structures, the r.m.s. deviations for C(alpha) atoms between hUBF HMG box 5 and HMG domains from Drosophila melanogaster protein D and Rattus norvegicus HMG1 are 1.5 and 1.6 A, respectively. This indicates that the differences between the crystal and NMR structures of hUBF HMG box 5 are larger than those with its homologous structures. The differences between the two structures potentially reflect two states with different structures. The specific interactions between the hUBF HMG box 5 and the first bromodomain of TBP-associated factor 1 (TAF1) were studied by ultrasensitive differential scanning calorimetry and chemical shift perturbation. Based on these experimental data, possible sites in hUBF HMG box 5 that may interact with the first bromodomain of TAF1 were proposed. Structure of human upstream binding factor HMG box 5 and site for binding of the cell-cycle regulatory factor TAF1.,Rong H, Li Y, Shi X, Zhang X, Gao Y, Dai H, Teng M, Niu L, Liu Q, Hao Q Acta Crystallogr D Biol Crystallogr. 2007 Jun;63(Pt 6):730-7. Epub 2007, May 15. PMID:17505112 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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