2w9p

CRYSTAL STRUCTURE OF POTATO MULTICYSTATIN

spinqualitylabelsall models 2w9p, resolution 2.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2w9p is a 14 chain structure with sequence from Solanum tuberosum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 2w9q Resources: FirstGlance, OCA, RCSB, PDBsum Function [CYTM_SOLTU] Probably has a role in the plant's defense system. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Potato (Solanum tuberosum) multicystatin (PMC) is a crystalline Cys protease inhibitor present in the subphellogen layer of potato tubers. It consists of eight tandem domains of similar size and sequence. Our in vitro results showed that the pH/PO(4)(-)-dependent oligomeric behavior of PMC was due to its multidomain nature and was not a characteristic of the individual domains. Using a single domain of PMC, which still maintains inhibitor activity, we identified a target protein of PMC, a putative Cys protease. In addition, our crystal structure of a representative repeating unit of PMC, PMC-2, showed structural similarity to both type I and type II cystatins. The N-terminal trunk, alpha-helix, and L2 region of PMC-2 were most similar to those of type I cystatins, while the conformation of L1 more closely resembled that of type II cystatins. The structure of PMC-2 was most similar to the intensely sweet protein monellin from Dioscorephyllum cumminisii (serendipity berry), despite a low level of sequence similarity. We present a model for the possible molecular organization of the eight inhibitory domains in crystalline PMC. The unique molecular properties of the oligomeric PMC crystal are discussed in relation to its potential function in regulating the activity of proteases in potato tubers. Characterization of Solanum tuberosum multicystatin and its structural comparison with other cystatins.,Nissen MS, Kumar GN, Youn B, Knowles DB, Lam KS, Ballinger WJ, Knowles NR, Kang C Plant Cell. 2009 Mar;21(3):861-75. Epub 2009 Mar 20. PMID:19304935 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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