2bgs: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:2bgs.gif|left|200px]] | [[Image:2bgs.gif|left|200px]] | ||
'''HOLO ALDOSE REDUCTASE FROM BARLEY''' | {{Structure | ||
|PDB= 2bgs |SIZE=350|CAPTION= <scene name='initialview01'>2bgs</scene>, resolution 1.64Å | |||
|SITE= <scene name='pdbsite=AC1:Binding+Site+For+Residue+Ndp+A+1321'>AC1</scene>, <scene name='pdbsite=AC2:Binding+Site+For+Residue+Bct+A+1322'>AC2</scene>, <scene name='pdbsite=AC3:Binding+Site+For+Residue+So4+A+1323'>AC3</scene> and <scene name='pdbsite=AC4:Binding+Site+For+Residue+So4+A+1324'>AC4</scene> | |||
|LIGAND= <scene name='pdbligand=BCT:BICARBONATE+ION'>BCT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=NDP:NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE'>NDP</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Aldehyde_reductase Aldehyde reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.21 1.1.1.21] | |||
|GENE= | |||
}} | |||
'''HOLO ALDOSE REDUCTASE FROM BARLEY''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
2BGS is a [ | 2BGS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BGS OCA]. | ||
==Reference== | ==Reference== | ||
An ABA and GA modulated gene expressed in the barley embryo encodes an aldose reductase related protein., Bartels D, Engelhardt K, Roncarati R, Schneider K, Rotter M, Salamini F, EMBO J. 1991 May;10(5):1037-43. PMID:[http:// | An ABA and GA modulated gene expressed in the barley embryo encodes an aldose reductase related protein., Bartels D, Engelhardt K, Roncarati R, Schneider K, Rotter M, Salamini F, EMBO J. 1991 May;10(5):1037-43. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1827067 1827067] | ||
[[Category: Aldehyde reductase]] | [[Category: Aldehyde reductase]] | ||
[[Category: Hordeum vulgare]] | [[Category: Hordeum vulgare]] | ||
| Line 27: | Line 36: | ||
[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:01:46 2008'' | ||
Revision as of 14:01, 20 March 2008
| |||||||||||||
| 2bgs, resolution 1.64Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Sites: | AC1, AC2, AC3 and AC4 | ||||||||||||
| Ligands: | BCT, SO4 and NDP | ||||||||||||
| Activity: | Aldehyde reductase, with EC number 1.1.1.21 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
HOLO ALDOSE REDUCTASE FROM BARLEY
Overview
In most higher plants a period of desiccation is the terminal event in embryogenesis. Excised barley embryos acquire desiccation tolerance at a precise developmental stage and cDNA clones have been isolated which are temporally linked with desiccation tolerance. One such clone (pG22-69) with a putative gene product of 34 kd displays high structural homology to mammalian genes encoding an NADPH dependent aldose reductase involved in the synthesis of sorbitol. This first aldose reductase gene of plants is expressed constitutively during embryo maturation and is modulated by the plant hormones abscisic acid (ABA) and gibberellic acid (GA). Immunohistochemistry showed that the protein is preferentially expressed in tissues formed at early stages in embryogenesis. Measurements of enzymatic activity indicate that pG22-69 encodes an active aldose reductase. The finding of this reductase activity and the cloning of the corresponding gene supports the existence of a metabolic pathway in plants playing a role in the synthesis of osmolytes like sorbitol. The significance of this work is that genes of related structure and functions are being used in diverse organisms to fulfil stress related biological requirements.
About this Structure
2BGS is a Single protein structure of sequence from Hordeum vulgare. Full crystallographic information is available from OCA.
Reference
An ABA and GA modulated gene expressed in the barley embryo encodes an aldose reductase related protein., Bartels D, Engelhardt K, Roncarati R, Schneider K, Rotter M, Salamini F, EMBO J. 1991 May;10(5):1037-43. PMID:1827067
Page seeded by OCA on Thu Mar 20 16:01:46 2008
